Background and objectives: Cardiopulmonary resuscitation (CPR) is a life-saving intervention which is casually performed by nurses. The nurses' knowledge and skills play a great role in examining the effect of educational training on nurses' performance on the guidelines in manipulating cardiopulmonary resuscitation. Materials and methods: In this experimental study we evaluated 66 nurses of the emergency Room (ER), ICU, CCU, and internal wards of Taleghani Hospital in Abadan, Iran in CPR before and after educational training. A validated researcher-made questionnaire was used since its validity and reliability were highly observed. It contained demographic information and included 20 items on nurses according to the Cardiovascular Recovery Guide 2016. All participants received an 8-hour training session and the scores were compared before, and one month after, educational intervention. The data were calculated through SPSS software version 16. Results: Data were collected among the 66 nurses who participated in the study. They were one male and 65 female nurses. Participants' age was 30.27±6.71 years and their work experience of 6.79±6.36 (1-27) years. The educational level of most participants was nursery including bachelor (92.4%) and most in ER (31.8%). Score of the nurses' was 35.24±2.45 at baseline and that increased to 38.07±1.58 after the educational intervention (p<0.001). That was significantly higher in participants who were above 30 years old than those who were less than 30 years old. Their age was correlated by the increasing years of their job experience and was different in nurses who worked in various wards (all p<0.001), but they did not differ in their educational level (p=0.13). Conclusion: The nurses of Taleghani Hospital had experience of CPR guidelines at the significant level which improved after the educational training; this issue showed the necessity of routine educational sessions on CPR for nurses.