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A B S T R A C TSorghum bicolor (L.) (Moench), which stands out for dry matter yield per hectare, has been considered as potential raw material for biofuels and electricity generation. It has a production cycle of six months, possibility of mechanization of cultivation and harvest and good adaptation to most regions of Brazil. Sorghum genotypes were evaluated for agronomic potential and chemical composition favorable to the production of second-generation ethanol. Three brown midrib (bmr) sorghum mutant hybrids were compared to three conventional hybrids. The bmr sorghum mutant hybrids are associated with reduced lignin content, making these genotypes more promising to the enzymatic conversion processes of the biomass. Sorghum biomass showed a high potential in terms of biomass production, with an average dry matter yield of 26.57 Mg ha −1 . Brown midrib sorghum hybrids showed significantly lower lignin contents than conventional hybrids and demonstrated the potential for cellulosic ethanol production.