Жила В. І. кандидат технічних наук, доцент, професор кафедри інтегрованих електротехнологій та енергетичного машинобудування Державний біотехнологічний університет м. Харків, Україна

The erythrocytes of human blood were sequentially irradiated with a low-intensity laser (λ = 640 nm), a violet LED (λ = 400 nm), a green LED (λ = 540 nm), and a yellow LED (λ = 592 nm). The method of acid (chemical) erythrograms and the method of counter ion transport compared the kinetic characteristics in irradiated and unirradiated blood samples. Received: - by the method of acid erythrograms it was found that in the irradiated blood samples there is a decrease in the time of hemolysis; - low intensity laser radiation, as well as the emission of LEDs, increase the rate of counter ion transport through red blood cells. - a decrease in the time of hemolysis and an increase in the rate of counter ion transport of irradiated blood samples is due to a decrease in the "effective" thickness of the near-membrane diffusion layer - an immiscible layer of water adjoined to the erythrocyte membrane. A decrease in the "effective” thickness of the near-membrane water layer (minimal in the wavelength range of 570-590 nm and 630-640 nm) changes the rate of metabolic processes in the "cell - intercellular medium" system, changing the mode of cell functioning. The altered mode of functioning is a biological response to light radiation. Red blood cells with an altered mode of functioning are signals - stimuli that cause the body to mobilize resources to fight pathology. These circumstances can predict the creation of a universal phototherapeutic equipment for extracorporeal blood irradiation based on light-emitting diodes with certain exposure parameters.

Modern technologies for the production of livestock products provide for long-term keeping of young animals indoors with an artificial microclimate. That is, it is expected to maintain within the required limits of temperature, humidity, gassiness, lighting level, etc. In particular, to compensate for solar radiation, ultraviolet irradiation units are used. The main criterion of UV irradiation is the dose, which is individual for each species and age of animals. The obtained amount of irradiation depends on the type and power of the radiation source, the height of the suspension, the continuance of the irradiation process. Therefore, existing mobile UV irradiators need to be upgraded for efficient operation. The purpose of the study is to develop a UV irradiation system for a room with pigs of different ages and weights in pens with the possibility of automatically providing the required dose. In order to increase the accuracy maintenance of the recommended dose of UV radiation, the authors use a video camera and a device with software that fulfils the effect of "pattern recognition" and automatic control of the installation during its movement over animals, which are kept in boxes in different age groups. Experimental studies showed that in the experimental groups of pigs, where irradiation was carried out using the developed unit of automatic control of the UV installation, the set of live weight in piglets was 20-25 % more than in the control group.

Erythrocytes were irradiated with an ultraviolet lamp of the VMT type (λ = 253.7 nm). The kinetic characteristics in irradiated and non-irradiated blood samples were compared by the acid (chemical) erythrogram method and by the counter transport method. Obtained: - by the method of acidic erythrograms it was found that in ultraviolet-irradiated blood samples there is a decrease in the time of half hemolysis.  - ultraviolet radiation increases the speed of counter movement of ions through erythrocyte membranes. - a decrease in the time of half hemolysis and an increase in the rate of counter transfer of ions, irradiated blood samples, is due to a decrease in the "effective" thickness of the near-membrane diffusion layer - an immiscible layer of water adjacent to the erythrocyte membrane. A decrease in the "effective thickness" of the near-membrane water layer changes the rate of metabolic processes in the "cell - intercellular medium" system, changing the mode of cell functioning. The altered mode of functioning is a biological response to light radiation. Red blood cells with an altered mode of functioning are stimulus signals that force the body to mobilize resources to fight pathology. These circumstances can predict the creation of a universal phototherapeutic equipment for extracorporeal blood irradiation based on LEDs with certain exposure parameters. Figs.: 4. Tabl.: 3. Refs.: 12 titles.