Ten faecal samples of bovine rotavirus from calves less than 30 days old from an outbreak of diarrhea in Hidrolândia, Goiás, Brazil were submitted to serological and molecular characterization, using enzyme immunoassay for subgrouping and serotyping, PAGE for determination of electropherotypes and PCR for genome typing. Nine samples belonged to group A/subgroup I rotavirus and one sample was group A / subgroup non-I/non-II. Four samples were characterized as G10P[11] (B223-like), four samples showed a mixture of two rotavirus strains (G6G10 and P[5]P[11]), one sample was characterized as G6P [11] and one sample was characterized only by G serotyping/genotyping, and did not react with any P primer used. Two electropherotypes were detected and both were present in the same animal. This study demonstrates that two different electropherotypes and/or serotypes of bovine rotavirus can circulate in the same outbreak.
INTRODUÇÃOA imunização contra a rubéola é feita com vacinas vivas atenuadas preparadas com diversas cepas de vírus, sendo, de um modo geral, muito satisfatórios os resultados obtidos, em todos os aspectos sob que possam querer analisar-se. Diversas pesquisas feitas no sentido de avaliar a eficiência das vacinas atualmente disponíveis 4,18,21,30 comparam os níveis de anticorpos inibidores da hemaglutinação e anticorpos neutralizantes conseguidos com o processo vacinal, com os que se desenvolvem na infecção natural. Raramente fazem referência aos anticorpos fixadores do complemento, considerando vários autores que estes não se formam na infecção vacinal 11,13,24,43 . Tal conceito não tem mais razão para aceitar-se, uma vez que, respeitando determinadas técnicas, é possível detectar a presença de anticorpos fixadores do complemento como resposta à vacinação contra a rubéola 19,30,36 .A finalidade da presente pesquisa é avaliar a eficiência da vacina Wistar RA 27/3,
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