Prevention of Salmonella contamination of poultry products requires detailed knowledge of the most important risk factors associated with its presence in the production system. The aim of this study was to identify the risk factors for Salmonella contamination in 44 broiler and 51 layer farms and determine the biofilm development capacity of the strains isolated. Then, glutaraldehyde (50% vol/vol), formaldehyde (37% vol/vol), and hydroxide peroxide (35% vol/vol) were applied to evaluate their capacity to remove Salmonella, biofilm and nonbiofilm strains, isolated from each risk factor in an artificial contamination test in field conditions. Samples of feces, dust, surfaces, meconium, delivery-box liners, water tank, water dispensers, litter, vectors (rodents, flies, and beetles), and surfaces of the slaughter trucks were taken throughout the rearing period. All samples were analyzed in accordance with ISO 6579:2002 (Annex D). To evaluate biofilm development, a screening method based on the fluorescence of Salmonella colonies on calcofluor agar plates was used. In the artificial contamination test, the chemical solutions were prepared at a concentration of 1.0% and applied at exact times (1, 15, or 60 min). Our results showed that the most important risk factors for Salmonella contamination were dust, surfaces, and feces. Moreover, day-old broiler chicks reached the farm highly contaminated, and wild carriers played an important role in the recirculation of Salmonella in laying hen houses. The serotype most commonly isolated from each risk factor was Salmonella enteritidis, and irrespective of the origin of different serotypes, around 50% were able to produce biofilm. Finally, the use of glutaraldehyde, formaldehyde, and peroxygen at a concentration of 1.0% in field conditions are inadequate for Salmonella elimination irrespective of the serotype, the biofilm development capacity, and the disinfectant contact time.

Spain launched its own strategy on bioeconomy in January 2016 aiming at boosting a bioeconomy based on the sustainable and efficient production and use of biological resources. It highlights global societal challenges related with agricultural and biotechnological sciences in Spain and the great dynamism of the private sectors involved, particularly the agri-food, biotech and biomass sectors. The targeted sectors are food, agriculture and forestry, conditioned by water availability. It also includes the production of those industrial bioproducts and bioenergy obtained from the use and valorisation of wastes and residues and other non-conventional sources of biomass, in a circular economy. The strategy also puts a focus on rural and coastal development through several uses and services linked to ecosystems. The capacity to generate know-how in this area and the promotion of public and private collaboration are important pillars in order to enhance existing value chains and to create new ones. The strategy is led by R&I and Agriculture, Food and Environment policy managers and largely supported at regional level too. The strategic objective is the maintenance of the bioeconomy as an essential part of Spanish economy to contribute to the economic growth by creating new jobs and fostering investments.

Highly pathogenic avian influenza (HPAI) is considered one of the most important diseases of poultry. During the last 9 years, HPAI epidemics have been reported in Asia, the Americas, Africa, and in 18 countries of the European Union (EU). For that reason, it is possible that the risk for HPAI virus (HPAIV) introduction into Spain may have recently increased. Because of the EU free-trade policy and because legal trade of live poultry was considered an important route for HPAI spread in certain regions of the world, there are fears that Spain may become HPAIV-infected as a consequence of the legal introduction of live poultry. However, no quantitative assessment of the risk for HPAIV introduction into Spain or into any other EU member state via the trade of poultry has been published in the peer-reviewed literature. This article presents the results of the first quantitative assessment of the risk for HPAIV introduction into a free country via legal trade of live poultry, along with estimates of the geographical variation of the risk and of the relative contribution of exporting countries and susceptible poultry species to the risk. The annual mean risk for HPAI introduction into Spain was estimated to be as low as 1.36 x 10(-3), suggesting that under prevailing conditions, introduction of HPAIV into Spain through the trade of live poultry is unlikely to occur. Moreover, these results support the hypothesis that legal trade of live poultry does not impose a significant risk for the spread of HPAI into EU member states.

Eggs and poultry meat are a common source of human salmonellosis. Contamination of poultry or poultry meat may occur throughout the production chain. Nevertheless, in Spain, noncontaminated broiler meat may be sold for human consumption from 2011. The aims of this study were as follows: (i) assess Salmonella detection from feces samples during the rearing and (ii) assess the influence of live transport to the slaughterhouse on Salmonella detection. During this study, 65 flocks were sampled at weekly intervals from the first day of rearing until slaughter. Samples of feces were taken from the litter using 5 pairs of cellulose sock swabs attached to boots and applied over the length of the house. To assess Salmonella detection rates before and after live transport to the slaughterhouse, feces samples were collected. Before loading, feces with 5 pairs of cellulose sock swabs were taken as described above. After transport, 2 pooled feces samples were taken directly from the truck (200 to 300 g each). All samples were analyzed in accordance with ISO 6579:2002 (Annex D). Results showed that regardless of whether broiler flocks arrived at the farm already shedding the bacteria in feces or they were infected during rearing, both groups described the same detection pattern, with the highest detection in feces at 14th day of rearing (50.5 and 34.5%, respectively). Moreover, Salmonella Enteritidis was the most prevalent serotype isolated during rearing (66.7%), followed by Salmonella Virchow (13.7%), Salmonella Hadar (9.4%), and Salmonella Ohio (2.8%). On the other hand, before loading and after transport to the slaughterhouse, 15.4 and 41.2% of feces samples collected were determined positive, respectively. In addition, a change in the serotype pattern was also observed. Salmonella Enteritidis remains the most prevalent serotype isolated (54.5%). Salmonella Hadar doubled the excretion rates (39.3%), and Salmonella Virchow and Salmonella Ohio were not isolated after transport.

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