This experiment was conducted to study the effects of different sources of zinc (Zn) on blood metabolites and balances of some minerals in lambs. In the first part, 20 6-7-month-old lambs were randomly allotted to four treatments including (1) basal diet containing 22.47 mg Zn/kg DM without supplementary Zn (control), (2) basal diet + 40 mg Zn/kg DM as ZnSO4 (ZnSO4 40), (3) basal diet + 20 mg Zn/kg DM as Zn-proteinate (Zn-Pro 20), and (4) basal diet + 40 mg Zn/kg DM as Zn-proteinate (Zn-Pro 40). Blood samples were taken on days 0, 28, and 65 before morning feeding. In the second part, four lambs from each treatment were randomly transferred to metabolic cages to evaluate the effects of different sources of Zn on N, Zn, Fe, and Cu retentions. This trial consisted of 18 days, with the first 12 days as the adaptation period followed by 6 days of sample collection. The results of this study showed that the source of Zinc had no significant effect on the analyzed parameters. Average daily gain and feed efficiency were improved by Zn supplementation (P < 0.05). Daily feed intake, plasma glucose, Fe and Cu concentrations, serum total antioxidant capacity, red blood cell count, packed cell volume, and hemoglobin concentration did not differ significantly between treatments (P > 0.05). Plasma Zn concentration, alkaline phosphatase (ALP) and bone-specific alkaline phosphatase (BALP) activity, and white blood cell and lymphocyte count differed significantly between control and Zn-supplemented groups (P < 0.05) as Zn supplementation improved these parameters. Nitrogen, Fe, and Cu retentions did not differ between treatments (P > 0.05). Zinc retention showed a significant difference between control and Zn-supplemented groups (P < 0.05), but there were no significant differences among the Zn-supplemented groups. The results of this study show that Zn supplementation improved performance and zinc retention in lambs. However, there were no significant differences between zinc sources used in this study.
Twenty four periparturient cows were used to determine the effects of DCAD on acid-base balance, plasma and urine mineral concentrations, health status, and subsequent lactation performance. Each group of 12 cows received either a diet containing −100 DCAD or +100 DCAD for 21 d prepartum. Both anionic and cationic groups were divided into two groups, one received a +200 DCAD and the other +400 DCAD diet for 60 d postpartum. Prepartum reduction of DCAD decreased DMI, urinary and blood pH, urinary concentrations of Na or K and increased plasma and urinary Ca, Mg, Cl and S. Also cows fed −100 DCAD diet consumed the most dry matter in the first 60 d after calving. Postpartum +400 DCAD increased milk fat and total solid percentages, urinary and blood pH and urinary Na and K concentrations, but urinary Ca, P, Cl and S contents decreased. Greater DMI, FCM yields were observed in cows fed a diet of +400 DCAD than +200 DCAD. No case of milk fever occurred for any diets but feeding with a negative DCAD diet reduced placenta expulsion time. In conclusion, feeding negative DCAD in late gestation period and high DCAD in early lactation improves performance and productivity of dairy cows.
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