Alzheimer's is the typical type of dementia that affects Senior people and is an irrevocable, progressive neurodegenerative disorder. The characteristic symptoms are loss of memory, behavioral disturbances, mood and personality swing, followed by diminished cognitive performance. There is no permanent remedy for AD and the existing drugs in the treatment of disease have minimal effectiveness. Medicinal plants used Ayurveda have been found more productive source for lead development of drugs, and many advanced herbal products are being evaluated are in the developmental stage in a clinical trial. In fact, several studies have reported the use of various Ayurvedic medicinal plant extracts and their most active chemical constituents for the management of Alzheimer's disease. Although the accurate molecular mechanism is unknown. Pipali and Jyotishmati are extensively used in Ayurveda for various diseases and for the enhancement of memory. An attempt has been made in the current study to acknowledge the activity of extracts of Piper longum and Celastrus paniculatus, and marker compounds like Piperine on acetylcholinesterase inhibition by in-vitro and Rivastigmin as standard. In-silico AChE inhibition studies were carried out on various active constituents like Piperine, Paniculatine, Malkanguinine and Donepezil as standard. Both the enhanced extracts and the standard marker have promising AChE inhibitory action, indicating that they could be used to treat memory problems.