In the Stranja Mountains of southeastern Bulgaria, native populations of Cicer montbretii Jaub. & Spach were found on the edge of a road in an oak forest near the village of Gramatikova (42°1′38″N; 27°36′49″E) at an elevation of about 125 m. C. montbretii, a perennial species, is the only wild Cicer sp. native to Bulgaria. At the time of collection, necrotic lesions were observed on the stems, leaflets, and pods of several plants, and these lesions were reminiscent of those induced by Ascochyta rabiei (Pass.) Labrousse. The teleomorph (sexual stage) of A. rabiei, Didymella rabiei (Kovachevski) v. Arx (syn. Mycosphaerella rabiei Kovachevski), was discovered in 1936 on overwintered chickpea residue in southern Bulgaria. The fungus is heterothallic and requires the pairing of two compatible mating types for development of fertile pseudothecia. Both mating types of A. rabiei were isolated previously from naturally infected, cultivated chickpeas (C. arietinum L.) from northeastern and southern Bulgaria (1), and the teleomorph, Didymella rabiei (Kovachevski) v. Arx, developed on naturally infested chickpea debris from both regions when it was incubated at appropriate environmental conditions. Isolations were made from lesions on the leaflets, stems, pods, and seeds of C. montbretii by surface disinfecting tissue in 0.25% NaOCl for 5 min, drying on paper hand towels, and placing small pieces of tissue on 2% water agar and Difco potato dextrose agar. Plates were incubated at 22 to 24°C under fluorescent lights with a 12-h photoperiod. A. rabiei was isolated from all foliar tissues of the plant, including seeds. Koch's postulates were fulfilled by inoculating the foliage of chickpea PI 458870 and reisolating the fungus from lesions that developed on the leaflets and stems. Six Bulgarian isolates of A. rabiei from C. montbretii were paired with compatible mating type tester isolates of A. rabiei, MAT1-1 (ATCC 76501) and MAT 1-2 (ATCC 76502), following the procedure of Kaiser and Kusmenoglu (2). Both mating types were found among the six isolates. Two were MAT 1-1 and four MAT 1-2. The teleomorph did not develop on the small amount of naturally infested chickpea residue tested. Therefore, in Bulgaria, both cultivated and wild chickpeas are infected naturally by A. rabiei and both mating types have been isolated from these hosts. D. rabiei will likely be found in native stands of C. montbretii in Bulgaria as more samples of overwintered infested debris are examined for the teleomorph. This is the first report of A. rabiei causing blight of a wild Cicer sp. References: (1) W. J. Kaiser. Can. J. Plant Pathol. 19:215, 1997. (2) W. J. Kaiser and I. Kusmenoglu. Plant Dis. 81:1284, 1997.

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In Developing Countries particularly in the West Asia and North Africa region, the major lentil breeding goals are development of cultivars with higher yields, resistance to major diseases, suitability for mechanical harvesting and improved residue yields because of the value placed on lentil straw as animal feed. By contrast, improved disease resistance, reduced tendencies to lodge, reduced pod and seed shatter, increased seed yield and improved seed quality traits are principal breeding goals in Developed Countries. Techniques of breeding lentil are similar to those used for other self-pollinating crops with some modifications.

In June 1992 and 1995, anthracnose of lentil (Lens culinaris Medik.) incited by Colletotrichum truncatum (Schwein.) Andrus & W. D. Moore was widespread in field trials at the Institute for Wheat and Sunflower ‘Dobroudja’ near General Toshevo in northeastern Bulgaria. Lesions on the leaves, stems, and pods were usually white to grayish on younger plants, often turning brown as plants matured. Severe infection usually resulted in dieback and/or death of plants. Acervuli containing spores and dark setae were observed within lesions, and conidia from the acervuli produced pure cultures of C. truncatum. Conidia were hyaline, onecelled, falcate to nearly straight with a prominent clear area in the center of highly granular cytoplasm, and measured 17.6 to 19.8 × 4.4 μm. C. truncatum was seed-borne in naturally infected lentil cv. Tadjikskaya 95 at low frequencies (<2%). Koch's postulates were fulfilled by inoculating the foliage of lentil cvs. Brewer and Pardina and reisolating the fungus from stem and petiole lesions. In pathogenicity tests, three isolates of C. truncatum from the foliage and seeds of lentil caused severe symptoms on inoculated lentil cvs. Brewer and Pardina, similar to those observed on diseased lentils in Bulgaria. The fungus also caused moderate symptoms on inoculated faba bean (Vicia faba L.) and pea (Pisum sativum L.), and light symptoms on inoculated chickpea (Cicer arietinum L.). In 1995, 258 USDA Plant Introduction (PI) accessions from the USDA lentil core collection were screened in replicated trials in northeastern Bulgaria and disease symptoms were observed in >90% of the lines. Anthracnose severity ranged from light to severe. A few accessions appeared to have acceptable levels of resistance to the disease. These included accessions from Iran (PI 431714 and 431717) and Spain (PI 533693). Also that year, C. truncatum was isolated from stem lesions of naturally infected bitter vetch (Vicia ervilia (L.) Willd.) at the Institute for Wheat and Sunflower ‘Dobroudja’. The disease in Bulgaria appears to be identical to one causing anthracnose of lentil in Canada (1) and the United States (2). This is the first report of C. truncatum causing anthracnose of lentil in Bulgaria. References: (1) R. A. A. Morrall. Plant Dis. 72:994, 1988. (2) J. R. Venette et al. Plant Dis. 78:1216, 1994.

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