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Considering environmental issues and to reduce dependency on fossil fuel many countries have politicized to replenish fossil fuel demand from renewable sources. Citing the potential of Jatropha mostly without any scientific and technological backup, it is believed to be one of the most suitable biofuel candidates. Huge grants were released by many projects for huge plantation of Jatropha (millions of hectares). Unfortunately, there has been no significant progress, and Jatropha did not contribute much in the energy scenario. Unavailability of high-yielding cultivar, large-scale plantation without the evaluation of the planting materials, knowledge gap and basic research gap seem to be the main reasons for failure. Thus, the production of Jatropha as a biofuel has been confronted with various challenges such as production, oil extraction, conversion and also its use as a sustainable biofuel. In this chapter, we disclose the challenges and possible remedy for the contribution in the biofuel industry.

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Plant attributes have direct and indirect effects on soil microbes via plant inputs and plant-mediated soil changes. However, whether plant taxonomic and functional diversities can explain the soil microbial diversity of restored forest ecosystems remains elusive. Here, we tested the linkage between plant attributes and soil microbial communities in four restored forests (Acacia species, Eucalyptus species, mixed coniferous species, mixed native species). The trait-based approaches were applied for plant properties and high-throughput Illumina sequencing was applied for fungal and bacterial diversity. The total number of soil microbial operational taxonomic units (OTUs) varied among the four forests. The highest richness of fungal OTUs was found in the Acacia forest. However, bacterial OTUs were highest in the Eucalyptus forest. Species richness was positively and significantly related to fungal and bacterial richness. Plant taxonomic diversity (species richness and species diversity) explained more of the soil microbial diversity than the functional diversity and soil properties. Prediction of fungal richness was better than that of bacterial richness. In addition, root traits explained more variation than the leaf traits. Overall, plant taxonomic diversity played a more important role than plant functional diversity and soil properties in shaping the soil microbial diversity of the four forests.