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-This study investigated the effect of organic loading rate (OLR) and sulfate loading rate (SLR) on landfill leachate treatment by a lab-scale anaerobic baffled reactor (ABR). Landfill leachate contained a concentration of organic matter between 3966 and 5090 mg COD.L -1 and no detectable amounts of sulfate. Reactors were started-up by feeding them with iron-sulfate at a SLR of 0.05 g SO 4 2-.L -1.day -1 (4 weeks). Factorial design and response surface techniques were used to evaluate and optimize the effects of these operating variables on COD removal. ABRs were operated at OLRs ranging from 0.30 up to 6.84 g COD.L with a COD/SO 4 -2 ratio of 40. Under these conditions sulfate is mainly used for molecular hydrogen consumption while organic matter is preferentially degraded via methanogesis.