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SUMMARYIn situ hybridization studies have been carried out on brain samples from eight cases of multiple sclerosis (MS) and 56 non-neurological and neurological controls, using single-stranded 35S-labelled RNA probes prepared against genomic RNA sequences of measles virus, canine distemper virus, rubella virus and simian virus 5. Foci of hybridization were found using probes against the measles virus nucleocapsid protein (N), phosphoprotein and fusion protein gene sequences in two of the MS cases, and also in one control, a case of disseminated cytomegalovirus infection with spinal cord necrosis. This result was confirmed using biotinylated probes prepared against the measles virus N genomic sequence. No hybridization was found in any of the MS or control cases using any of the other viral genome-specific probes.

The peritoneal equilibration test (PET) is a standardized procedure to assess peritoneal membrane performance in patients on continuous ambulatory 2 Cook JGH. Factors influencing the assay of creatinine.

A series of aminoaromatic derivatives of diethylenetriaminepentaacetic acid (DTPA) has been prepared, in a search for terbium chelates suitable for use in fluorescence immunoassay. Most of the derivatives contained heterocyclic rings with at least one nitrogen atom. The fluorescence properties of the terbium chelate of each compound were examined. Although none of the products proved suitable for use in immunoassays, the terbium chelate formed from the product of the reaction between DTPA anhydride and cytosine (4-amino-2-hydroxypyrimidine) was particularly fluorescent and had a long fluorescence lifetime. It was unstable, however, in aqueous solution below pH 9. The fluorescence properties of some europium complexes were also examined.