No abstract

Among the causes that cause significant economic damage to the rabbit industry are parasitic and infectious diseases. In different geographical areas, most protozoan and helminthic diseases exist as monoparasitosis and various associations. Associative invasions occur in various combinations. There is not enough information in the literature that could give a complete picture of the current state of infections and invasions of the gastrointestinal tract of rabbits in Ukraine. The aim of our work was to establish the epizootic situation regarding the pathogens of invasive and infectious diseases of the gastrointestinal tract of rabbits. The work was performed during 2019–2020. Monitoring of the epizootic situation regarding parasitosis of the gastrointestinal tract of rabbits was carried out based on the results of coproscopic studies of animals from different regions of Ukraine. The spread of the disease was established by clinical observation, helminthoscopic, helminthovoscopic, helmintholarvoscopic studies of animals and incomplete helminthological autopsy of rabbits aged from birth to 4 years. According to our research, it was found that spirochetosis as a monoinfection was registered in 3.32 % of animals, eimeriosis as a monoinvasion – in 10.70 %, passalurosis – in 3.33 %, strongyloidosis – in 4.79 %, cysticercosis – in 1.85 %. Parasitosis of the gastrointestinal tract of rabbits most often occurred in the form of myxinvasions (76.01 %). Most often associative parasitosis of rabbits was detected as part of two-component mixinvasions (54,85%), less often recorded in the form of three-component mixinvasions (37.38 %) and four-component mixinvasions (7.77 %). Polyinvasions consisted of associations: prokaryotes, protozoa and nematodes (32.52 %), protozoa and nematodes (30.10 %), different types of nematodes (11.17 %), prokaryotes and nematodes (9.22 %), prokaryotes and protozoa (6.80 %), protozoa and cestodes (3.88 %), cestodes and nematodes (2.91 %), prokaryotes and cestodes (2.43 %), prokaryotes, protozoa, cestodes and nematodes (0.97 %), protozoa, cestodes and nematodes (0.49 %). In the parasitocenoses of the gastrointestinal tract of rabbits are most often registered in the form of associations with Eimeria sрp. and Treponema cuniculi (19.42 %), Eimeria sрp., Passalurus ambiguus and Treponema cuniculi (18.45 %), Eimeria sрp., Treponema cuniculi and Strongyloides papillosus (13.11 %), Passalurus ambiguus and Strongyloides papillosus (11.17 %). In total, 11 combinations of different types of parasites were identified, the co-members of which are passalurus, 10 – eimeria, 7 – strongyloides and treponema, 6 – cysticercus.

Biochemical parameters of rabbit blood are important for the control of animal diseases and the impact on meat productivity of diets and various supplements. The biochemical profile of blood changes under the influence of various factors, including temperature and humidity, which are associated with seasonality. The aim of the study was to determine the influence of seasonality on the biochemical analysis of blood of meat rabbits.The average annual temperature in the rabbitry was 15.9±1.10 °? during the observation period, with minimum values in winter +5.30 °?, maximum values in summer +27.70 °? and an average relative humidity of 68.3±1.2%. The seasonal fluctuations (within physiological values) of some serum indicators of protein-nitrogen metabolism in males of Californian rabbits breed have been established. In spring, we observed a significant increase in the concentration of total protein by 7.0-12.0% and albumin – by 9.7-9.8%. At the same time, the lowest values of urea, creatinine and uric acid were equal or less than the average annual physiological indicators. Deviations of temperature from comfortable indicators in winter and summer led to the adaptive changes in rabbit organisms. We revealed the changes in the creatinine econtent in summer, uric acid content in summer and winter and increased activity of ALAT enzyme in winter and summer. We suggested that in case of rabbit keeping in rooms without artificial regulation of the microclimate, it is necessary to consider the influence of seasonal temperatures on blood serum biochemical parameters.

Дніпропетровський державний аграрно-економічний університет М. П. Прус, доктор ветеринарних наук, професор ORCID ID: 0000-0002-6879-1561 Національний університет біоресурсів і природокористування України У крові кролів всіх дослідних груп за впливу збудника Passalurus ambiguus, у порівнянні з аналогічними показниками здорових тварин, виявили достовірно високі рівні (p<0,001) вмісту загального протеїну, глобулінів, γ-глобулінів, креатиніну, IgA, IgG, IgM і В-лімфоцитів на фоні низьких рівнів сечової кислоти та протеїнового коефіцієнту. Вірогідно вищими були вміст β-глобулінів і α2-глобулінів у крові тварин з середнім і високим рівнем інтенсивності пасалурозної інвазії.

The concentration of chemical ements (Pb, Cd, Cu, As, Zn, Hg, Fe, Co, Mn) in the liver of healthy cattle and those affected by Fasciola hepatica and Dicrocoelium lanceatum in Poltava region (central part of Ukraine) was determined. The research was carried out by the method of atomic and absorption spectrometry carried out at the Regional State Laboratory of Veterinary Medicine in Poltava region. The liver samples (n = 30) from healthy cattle black-and-white breed and those affected by F. hepatica and D. lanceatum were taken at the meat processing plant. The ages of the cattle ranged from 6 to 8 years. The samples were immediately cooled, transported to the laboratory and stored at –20 °C for further analysis. The results of the research determined the average indicators of concentration of some toxic elements in the livers of healthy cattle and those infected by the trematodes. The content of chemical elements in the liver of healthy animals and those affected by fasciola can be represented in the form of a decreasing rank number: Zn > Fe > Cu, and for dicrocoeliosis, respectively, Fe > Zn > Cu. It has been established that Cu and Zn are involved in the metabolic processes of the body of trematodes, which is confirmed by our research. The presence of F. hepatica and D. lanceatum in the body of cattle significantly reduces the level of copper and zinc, with a high inverse correlation dependence on the intensity of infection, thus indicating the possibility of their accumulation by helminths. Concentration of Cu and Zn in the liver of cattle with fasciolosis was 6.82 ± 0.29 and 35.77 ± 1.93 mg/kg, while for animals with dicrocoeliosis it was 3.90 ± 0.25 and 41.91 ± 2.22 mg/kg. The content of cobalt and manganese in the liver of healthy animals was, respectively, 0.05 ± 0.01 and 1.95 ± 0.06 mg/kg. In the case of Fasciola parasitising in the liver tissue, the level of cobalt (0.10 ± 0.02) and manganese (2.55 ± 0.16) significantly increased, positively correlating with the intensity of the infection, indicating no effect on the exchange and bioaccumulation of these elements by helminths.