The sea urchin embryo is a classical model system for studying the role of the cytoskeleton in such events as fertilization, mitosis, cleavage, cell migration and gastrulation. We have conducted an analysis of gene models derived from the Strongylocentrotus purpuratus genome assembly and have gathered strong evidence for the existence of multiple gene families encoding cytoskeletal proteins and their regulators in sea urchin. While many cytoskeletal genes have been cloned from sea urchin with sequences already existing in public databases, genome analysis reveals a significantly higher degree of diversity within certain gene families. Furthermore, genes are described corresponding to homologs of cytoskeletal proteins not previously documented in sea urchins. To illustrate the varying degree of sequence diversity that exists within cytoskeletal gene families, we conducted an analysis of genes encoding actins, specific actin-binding proteins, myosins, tubulins, kinesins, dyneins, specific microtubule-associated proteins, and intermediate filaments. We conducted ontological analysis of select genes to better understand the relatedness of urchin cytoskeletal genes to those of other deuterostomes. We analyzed developmental expression (EST) data to confirm the existence of select gene models and to understand their differential expression during various stages of early development.

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A total of 188 yearling steers of predominantly Angus and Hereford breeds, with mean body weight of 299 kg, were used in this study, which started on 8 April and finished on 3 October, to assess the effects of environmental factors on feed intake of steers in various housing systems. Housing consisted of outside lots with access to overhead shelter, outside lots with no overhead shelter and a cold confinement building. Ad libitum corn, 2.27 kg of 35% dry matter whole plant sorghum silage and 0.68 kg of a 61% protein-vitamin-mineral supplement was offered. Feed that was not consumed was measured to determine feed intake. The temperature data were recorded by hygro-thermographs. Hourly temperatures and humidity were used to develop weather variables. Regression analysis was used and weather variables were regressed on dry matter intake (DMI). When addition of a new variable did not improve R (2) more than one unit, then the number of variables in the model was truncated. Cattle in confinement had lower DMI than those in open lots and those in open lots with access to an overhead shelter (P < 0.05). Cattle in outside lots with access to overhead shelter had similar DMI compared to those in open lots (P = 0.065). Effect of heat was predominantly displayed in August in the three housing systems. In terms of explaining variation in DMI, in outside lots with access to overhead shelter, average and daytime temperatures were important factors, whereas in open lots, nocturnal, peak and average temperatures were important factors. In confinement buildings, the previous day's temperature and humidity index were the most important factors explaining variation in DMI. Results show the effect of housing and weather variables on DMI in summer and when considering these results, cattle producers wishing to improve cattle feedlot performance should consider housing conditions providing less stress or more comfort.

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Observations on the effects of season, housing and diet were made on 916 steers in three winter and two summer trials. Diets consisted of corn grain and corn silage, in balanced rations, fed ad libitum with energy ratios of 25:75 (Diet 1), 55:45 (Diet 2) and 85:15 (Diet 3). There were three types of housing systems: outside lots without access to overhead shelter (NS), outside lots with access to overhead shelter (OS) and an open-front confinement building (C). Average ambient temperatures and precipitation for winter and summer trials were -1.0 and 15.3 C and 4.67 and 10.81 cm/mo, respectively. Steers gained more (P less than .05) in summer than in winter. Within housing system, OS and NS steers gained faster and consumed more dry matter (DM) and energy (P less than .05) than C steers; C-fed steers were less (P less than .05) efficient (kg feed DM/kg gain) than OS steers. Steers fed Diet 1 had lower (P less than .05) average daily gain (ADG) than those fed Diets 2 and 3. Steers on Diet 3 consumed less DM (P less than .05) than those on Diets 1 and 2. Estimated metabolizable energy intake (MEI) was significantly less for cattle fed Diet 1 than those fed Diets 2 or 3. Diet 3 was more (P less than .05) efficient than Diet 1. Season x diet (P less than .10) and season x housing (P less than .10) interactions were found for daily DM intake and MEI. This resulted in greater cattle growth rate responses to higher grain diets in summer than in winter and more pronounced adverse effects of confinement rearing in summer than in winter. No evidence was found of other two-way or three-way interactions for any of the performance characteristics studied. These results indicate that in addition to important singular effects of season, housing and diet, important interactions of these factors also exist.