A relatively stable specific complex of the chromatin core histones H2A, H2B, H3, and H4 has been obtained in 2 M -NaCl/25 mM sodium phosphate buffer, pH 7.0. The histone core complex has an apparent specific volume of 0.73 ml/g. Its sedimentation coefficient was dependent on rotor speed (angular velocity, w) and attained different stable values at low and high rotor speeds. The drop in sedimentation coefficient occurred sharply between w2 values of about 9 X 106 and 1

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