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Field experiment was conducted at farmer’s field in Coimbatore District of Tamil Nadu with two different soil sulphur status i.e., soil with sufficient and deficient levels of sulphur status in order to study the response of graded doses of sulphur on two different sesamum varieties. The experiment was laid out in split plot design with two factors viz., Two sesamum varieties (TMV 7 - Black var., SVPR 1 - White var.) and Six sulphur levels (0, 20, 30, 40, 50, 60 kg S ha-1). Application of 30 and 50 kg S ha-1 at sulphur sufficient and sulphur deficient soils has increased the plant height at VS and FS, at harvest the highest plant height was recorded. Whereas, other important traits like number of branches per plant, number of capsules per plant, length of capsule and dry matter accumulation has registered the highest response for sulphur application at 30 kg ha-1 which was on par with higher doses in sulphur sufficient soil and in case of deficient soil significant response was observed till 50 kg S ha-1. Among the two varieties, TMV 7 has performed better when compared with SVPR 1.