This study was carried out on the preservation of fermented Parkia bigl from Local Producers and laboratory produced. The local and fermented laboratory controlled samples were treated with 0.5ml of freshly prepared ginger extract. Another set of control was left untreated. The samples were stored at ambient temperature for 30 and 120 days. Contaminant isolates were identified from locally produced, laboratory treated and untreated (Control) using standard procedure. The analysis consisted of Aerob Anaerobic mesophilic count, Staphylococcus count and f aureus, Salmonella sp, Mucor sp and Rhizopus sp. The proximate analysis and organoleptic assessment of the purchased, Laboratory treated and laboratory untre carried out using standard procedure at 30 and 120 days. bacterial isolates at 30 and 120 days in the treated and control locust bean cake were E.coli only isolated in Kwanar yandaddawa, purchased Locust bean cake, Salmonella sp was not detected and a predominant fungal species were Mucor and Rhizopus. There was log reduction between 30 and 120 days treated locust bean cake; the Control shows Log increase. The m percentage differences between 30 and 120 days shows nutritional quality of treated locust bean cake. Organoleptically, the judges rejected control daddawa as a result of what the panelist termed as "unpalatable taste and partiall growing interest in use of natural products of plant origin therefore gives Ginger extract an advantage in developing a practical approach for the preservation of African locust bean cake.
Hazard analysis and critical control point (HACCP) was carried out during the production of African locust bean seeds condiment (Daddawa) in a location that specialized in it's manufacturing namely Kwanar-yandaddawa (Dawakin-Tofa Local Government Area) Kano State, Nigeria. The analyses consisted of determination of the aerobic, anaerobic mesophilic bacterial, staphylococcal and fungal counts. In addition, detection of E. coli 0157: H7, Staphylococcus aureus and Clostridium perfringens on the raw seeds before processing and the processed daddawa were also carried out. The raw seeds, processed seeds, production stages and additives had high total microbial count in the range of 10 9-10 11 cfu/g, which is above the maximum acceptable limit of 10 5 cfu/g. Bacteria isolated and biochemically characterized were Staphylococcus spp, Clostridium perfringens and E. coli 0157: H7. Mucor and Rhizopus were the fungal genera frequently isolated from most of the samples with Aspergillus fumigatus least isolated. Clostridium botulinum was not isolated at Kwanar yandaddawa. The raw seeds, sand used in dehauling, diluents, pawpaw leaves used as additives as well as final molding, comparing (tabletting) and display for sale to consumers are therefore regarded as a critical control points.
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