Paddy straw mushroom/ Chinese mushroom (Volvariella volvacea) is highly suited in tropical climates. The success of this mushroom production is solely depended on high quality spawn and improved technologies. Among the additives, calcium carbonate amended beds recorded maximum amount of total glutathione, total phenol, flavonoids, lycopene, total carotenoids, vitamin A and vitamin C were recorded. This was followed by horse gram, gypsum and calcium carbonate + gypsum. In non-amended medium significantly very less amount of antioxidative substances were recorded. K e y w o r d sVolvariella volvacea, Antioxidant properties, Additives

An effort has been made to find the best medium and operating conditions for the production of extracellular L-asparaginase by Aspergillus terreus in shake culture fermentation through stepwise optimization strategies like studies on the effect of pH and temperature on L-asparaginase, studies on the enzyme activity in various concentration of L-glutamine and study the growth kinetics for L-glutamine medium. Our study shows that L-glutamine combined with L-asparagine influence the asparaginase production level and physical parameter such as pH and temperature has more contribution in enzyme production. The pH 8 and temperature 35°C found to be good environmental condition the production of extracellular L-asparaginase by Aspergillus terreus.

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