Out of 200 clinical samples (blood ,pus,stool,urine,wound and vaginal swabs) were collected from Baghdad hospitals during June2009-January 2010,96 Gram negative and positive bacterial isolates were identified by bacteriological and biochemical tests. Escherichia coli was the dominant bacteria (27.08%), followed by Salmonella typhi (12.5%), Klebsiella pneumoniae (10.4%), Proteus vulgaris (9.3%), and Staphylococcus aureus (8.3%).Antimicrobial susceptibility test was done against ciprofloxacin disk (5μg) by disk diffusion method and the resistant isolates were 22 (23%);distributed into Escherichia coli (10 isolates), Klebsiella pneumoniae (4 isolates), Salmonella typhi (3 isolates), Enterobacter and Shigella flexnerii (2 isolates) and Staphylococcus aureus one isolate. Seven ciprofloxacin resistant isolates were positive for β-lactamase production test (7.3%). Results of minimal inhibitory concentration of ciprofloxacin for positive β-lactamase producing isolates, E. coli 1, Enterobacter 1, Enterobacter 2, K. pneumoniae, S. flexneri, E.coli 2, and S.aureus were (64,32,32,16,16,16,8 μg/ml) respectly