Microbial anti-cancer enzymes have been proven to be effective and economical agents for cancer treatment. Aeromonas veronii has been identified as a microorganism with the potential to produce L-glutaminase, an anticancer agent effective against acute lymphocytic leukaemia. In this study, a selective medium of Aeromonas veronii was used to culture the microorganism. Strain improvement was done by adaptive and induced mutational techniques. A selective minimal agar media was incorporated for the growth of the strain which further supports adaptive mutation. Strains were also UV-irradiated and successively treated with N-methyl-N'-nitro-N-nitrosoguanidine to find a resilient strain capable of producing L-glutaminase efficiently. The Plackett-Burman design and central composite designs were used to screen and optimize additional carbon and nitrogen sources. Adaptive mutation resulted in promising yield improvements compared to native strain (P<0.001). The mean yield of 30 treated colonies from the induced mutation was significantly increased compared to the non-induced strain (P< 0.001). The economically feasible statistical designs were found to reinforce each other in order to maximize the yield of the enzyme. The interactions of nutrient factors were understood from the 3D response surface plots. The model was found to be a perfect fit in terms of maximizing enzyme yield, with the productivity improving at every stage to a fourfold output of enzyme (591.11 ±7.97 IU/mL) compared to the native strain (135±3.51 IU/mL).
Soil samples were collected from the coastal region of Neendakara, along the West cost of Kerala, India. 15 fungal species were isolated and identified by using lacto phenol cotton blue staining method. From this, Aspergillus flavus was tested in Starch Hydrolysis Agar Medium for its amylase enzyme production under sumerged aerobic fermentation with different physico- chemical properties of substrates. Cocos nucifera meal was used as a carbon source. Heavy metals were added to these medium and were used as a modified medium. The effect of different carbon source, nitrogen compound and physico-chemical conditions like temperature, pH and incubation periods were studied for derivation of amylase enzyme. The molecular weight of enzyme was determined by SDS –PAGE. The role of heavy metals was determined by ion exchange chromatography. Cocos nucifera meal medium with dextrose has shown the highest amylase production at pH 6.0 and temperature of 30ºC with protein content of 201μg/ml; 98.4μg/ml and dry biomass of 1.28μg/ml. Keywords: Soil samples; A. flavus; amylase; SDS-PAGE; Ion exchange chromatography; submerged aerobic fermentation DOI: 10.3126/kuset.v6i2.4015Kathmandu University Journal of Science, Engineering and Technology Vol.6. No II, November, 2010, pp.75-87
Five coastal locations on the southeast coast of India severely disturbed after the tsunami on December 26, 2004 were surveyed for the occurrence of filamentous fungi on woody debris by means of short-term (1 month) and long-term (12 months) damp incubation. Short-term incubation revealed 26 mitosporic fungi (8 genera) ranging from 14 to 17 taxa per location with a total frequency of occurrence between 0.4 and 5.6 %. Aspergillus taxa were dominant and six of them were common to all locations. Long-term incubation yielded 35 fungi (25 genera) (22 ascomycetes, 1 basidiomycete and 12 mitosporic fungi). The total fungal taxa per location ranged from 25 to 28 with a total frequency of occurrence of 0.8 to 46.8 %. Corollospora gracilis was the most dominant ascomycete (46.8 %), while Cirrenalia tropicalis and Dictyosporium pelagicum dominated among mitosporic fungi (18 %). The total frequency of occurrence of 11 ascomycetes and four mitosporic fungi was above 10 %. Twelve ascomycetes and two mitosporic fungi were common to all locations. The richness of fungi was higher after long-term than short-term incubation. The occurrence of 61 fungal taxa in this study suggests that the tsunami-dumped woody litter on the southeast coast of India might have at least partially originated from the ocean, thus representing a mosaic of fungi existing in seawater and sediments.
Bagali tank is a perennial artificial water body situated 10 kms away from Harapanahalli to north-eastern region. The area of the tank is 146.2 hectares and depth is about 30 feet. The water body is irregular in shape. It is located at 14.842°N latitude and 75.989°E longitude. Rainwater, sewage and seepage from hilly regions is the main source of water to this tank. The water is used for agricultural practices and domestic activities. Due to anthropogenic activities, rapid industrial growth, domestic and agricultural activities of the region, the tank water is being polluted, which is the case with almost all major tanks of the region and also in India. A yearlong study was conducted to measure various physico-chemical and bacteriological parameters including levels of phytoplankton in the tank. The study revealed that there is indication of pollution in the Bagali Tank and hence preventive measures are required to avoid further deterioration of the tank water quality.
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