Aspectos da biologia e comportamentais de Gyropsylla spegazziniana (Lizer y Trelles) em condições de laboratório, foram estudados. O período de desenvolvimento do ovo à emergência do adulto foi de 38,7 dias, a longevidade do macho foi de 43,7 dias e das fêmeas foi de 15,2 dias. Ovos, ninfas e adultos foram descritos e ilustrados. ABSTRACT Biological aspects and behaivour of Gyropsylla spegazziniana (Lizer y Trelles), under laboratory conditions, were studied. Mean development time of immature stages to immago was 38,7 days; the longevity of the male was 43,7 days and the female was 15,2 days. Eggs, nymphs and adults are described and illustrated. RÉSUMÉ Ce travail se rapporte aux aspectes du comportement et de la biologie de Gyropsilla spegazziniana (Lizer y Trelles) au laboratoire. Le temps de développement, oeuf jusqu´ a l´occurrence du adulte a été 38,7 jours, la longévité des mâles a été 43,7 jours et des femelles a été 15,2 jours. Oeuf, nymphes et adultes sont décrites et ilustreés.
O objetivo deste trabalho foi avaliar a patogenicidade de isolados de Verticillium lecanii, sobre Cinara atlantica, e estimar a CL50 do melhor isolado. No teste de seleção, utilizaram-se mudas de pinus com ninfas do pulgão, 20 isolados do fungo e um controle, num total de 21 tratamentos e dez repetições. Na CL50 do isolado CG 904, utilizaram-se seis concentrações, com dez repetições. VL 6, CG 902 e VL 2 apresentaram os mais altos índices de mortalidade, 72,22%, 67,34% e 67,31%, respectivamente. A testemunha apresentou mortalidade de 15,6%. Nos bioensaios de CL50, a concentração de 10(8) conídios mL-1 , do isolado CG 904, causou mortalidade de 100%, CL50 de 2x10(5) conídios mL-1 e TL50 de 4,4 dias.
ResumoHedypathes betulinus (KLUG, 1825) (Coleoptera: Cerambycidae) é a principal praga da cultura da ervamate. Para o seu controle, avaliou-se em laboratório e campo a utilização de fungos entomopatogênicos. Em laboratório, foi avaliada a infectividade de isolados dos fungos Beauveria bassiana (Bals.) Vuillemin, 1912; B. brongniartii (Sacc.) conídios/mL, variando de 6x10 5 a 5x10 6 conídios/mL. Foi avaliada a persistência do isolado CG 716 de B. bassiana formulado à base de óleo emulsionável em campo, verificando-se alta mortalidade nas primeiras três semanas após a aplicação do fungo, variando de 95 a 78%, decaindo para 65 a 47%, um e dois meses após a aplicação, respectivamente. Palavras-chave: Controle biológico; broca-da-erva-mate; CL 50 . Abstract Entomopathogenic fungi in the control of Hedypathes betulinus and evaluation of persistence.Hedypathes betulinus (KLUG, 1825) (Coleoptera: Cerambycidae) is the main pest of "erva-mate" (Ilex paraguariensis) culture. Focusing its control, it was evaluated in laboratory and field the entomopathogenic fungi using. In laboratory, it was evaluated the infectiveness of the strains of the following fungi: Beauveria bassiana (Bals.) Vuillemin, B. brongniartii (Sacc.) Petch, Metarhizium anisopliae (Metsch.), Sorokin and Paecilomyces (= Isaria) sp. Bainier, in adults specimen of H. betulinus. The insects were immersed in a conidial suspension at a concentration of 10 7 conidia mL -1 . After the application the insects had been kept at laboratory for mortality register. It was verified that B. bassiana (CG 716) was more infective, reaching to 100% of mortality. It estimated LC 50 of isolated CG 716 of B. bassiana with five concentrations and a variation of l0 5 to l0 9 conidia mL -1, along the control plot. The estimated LC 50 was 2,0 x 10 6 conidia mL -1 , ranging from 6 x 10 5 to 5 x 10 6 conidia mL -1. The persistence of strain CG 716 of B. bassiana -formulate oil was evaluated in field, producing the highest mortality, at the first three weeks after the application of the fungus, with a variance of 95 to 78 %, with a decreasing of 65 to 47% , along one and two months after the application , respectively.
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