Alonso, A., Gomes, M.P.D., Martins, M.A. and Sondahl, M.S., 1990. Detection of foot-and-mouth disease virus infection-associated antigen antibodies: comparison of the enzyme-linked immunosorbent assay and agar gel immunodiffusion tests. Prev. Vet. Med.,.A liquid-phase enzyme-linked immunosorbent assay (ELISA) was compared with the standard agar gel immunodiffusion test (AGID) to identify and quantify antibodies against foot-and-mouth disease (FMD) virus infection-associated (VIA) antigen. A total of 3181 cattle sera were tested. Of these sera, 1885 were from cattle which had not been exposed to FMD. A total of 1296 sera were either from cattle which were experimentally exposed to FMD virus or from cattle involved in field outbreaks. The results indicate that the ELISA has the same specificity as the AGID test, but is more efficient in detecting cattle exposed to FMD virus. The ELISA technique will probably prove to be a more satisfactory test in support of the prevention, control and eradication programs for the disease.

An indirect "sandwich" enzyme-linked immunosorbent assay (ELISA) using polyvalent and monovalent antisera was compared with the 50% complement fixation (CF50) test for the detection of foot-and-mouth disease (FMD) O, A, and C virus types. ELISA was more sensitive than CF50 tests when polyvalent antisera were used for detecting the 3 types of virus in epithelial samples, whereas ELISA using monovalent antisera was the least sensitive technique. The ELISA performed with polyvalent antisera was 9 times more sensitive for detecting FMD virus than that with monovalent antisera. However, viral isolation in cell culture was the most sensitive detection system. The combined use of ELISA with polyvalent antisera and cell culture inoculations was the most effective procedure for identifying FMD virus in epithelial samples from the field.

Abstract. An indirect sandwich enzyme-linked immunosorbent assay (ELISA) has been used for vesicular stomatitis virus (VSV) typing using sets of monovalent and polyvalent rabbit/guinea pig antisera for identification of VSV types New Jersey (VNJ) and Indiana (VIND). The VIND polyvalent antiserum (VIND-P) detects any strain of the 3 subtypes of the VIND type (VIND-1, VIND-2, and VIND-3) with the same strong reactivity. It is also possible to subtype the VIND strains using VIND-P rabbit antiserum as capture antibody and monovalent VIND-1, VIND-2, or VIND-3 guinea pig antisera as detector. The ELISA proposed has about 10 times more sensitivity and provides 10% more positive results than does the complement fixation 50% (CF,,) test when epithelial samples are tested. Monovalent antisera were prepared against the following strains from the PAFMDC virus collection: VNJ Costa Rica./ and in pigs, VS must also be differentiated from Swine 66, VIND-1 Costa Rica/72, VIND-2 Ribeirao-Br/79, and vesicular disease and vesicular exanthema of swine. 18 VIND-3 Agulhas Negras-Br/86. Rabbit antisera (for capture) Vesicular stomatitis virus (VW) is a rhabdovirus were prepared with virus propagated in IB-RS-2 cells. 8 Tissue represented by serotypes New Jersey (VNJ) and In-culture fluids were concentrated 50 times with 8% w/v polydiana (VIND). 9 VIND has been subdivided into 3 sub-ethylene glycol, 6,000 mW, and were emulsified with equal tvpes: 11 VIND-1 identified in the endemic areas: VIND-volume of Freund's incomplete adjuvant (FIA). Rabbits were 2 isolated sporadically in Trinidad, 16 Argentina, 14 and Brazil; 3 and VIND-3 reported in Brazil. 3,5 Differential diagnosis of VS and FMD in cattle and pigs requires an accurate and rapid laboratory diagnosis. Traditionally, the complement fixation test (CF) has been used. 15 An indirect sandwich enzyme-linked immunosorbent assay (ELISA) for the laboratory diagnosis of VS that effectively detects VNJ and VIND-1 has been developed. 13 However, that test has low reactivity for strain subtypes VIND-2 and VIND-3. Vesicular stomatitis (VS) is an infectious vesicularThe purpose of the present study was to develop an ELISA and to evaluate its specificity and sensitivity to identify VNJ, VIND-1, VIND-2, and VIND-3 directly from field samples and from material amplified in cell culture. Results were compared with those of the complement fixation 50% (CF,,) test used at the Reference From the Pan American Foot-and-Mouth Disease Center,

Monoclonal antibodies (MAbs) were produced against foot-and-mouth disease (FMD) virus types O1 Campos Br1/58, A24 Cruzeiro Br1/55, and C3 Indaial Br1/71, which are the strains used for production of FMD vaccines in the majority of South American countries. Within the library of MAbs produced, a group was selected on the basis of their neutralizing titer in cell culture, protective titer in suckling mice, sensitivity to trypsin, and specificity for virus structural proteins. The MAbs were utilized in an ELISA test format to compare European and South American representative field isolates with vaccine production strains in their r1 relationship as obtained by 50% complement fixation (CF50) with polyclonal antibodies (PAb) and their virus neutralization (VN) relationship obtained with sera from one-time-vaccinated and from revaccinated cattle, respectively. The MAbs selected varied in their reactivity against the different strains and, therefore, enabled us to compare field FMDV strains to those against which the MAbs were produced, with definite advantages over the r1 and VN ratios. Thus, panels of MAb produced with the vaccine strains and appropriately selected are significantly useful for the FMD-control programs because they serve to provide guidance on the immunological coverage provided by the vaccines against FMDV strains circulating in the field. The MAbs are also useful for the differentiation of FMD virus strains.

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