The ethanolic extract of sugar beet pulp at 100% exibited good antioxidant activity of 69.74%. It showed an excellent scavenging activity on 1,1-diphenyl-2-picrylhydrazyl radicals (84.94%) and was a mild chelator for ferrous and cupric ions. On the basis of some identified phenolic acids in ethanolic extract from sugar beet pulp it is concluded that predominant acids (ferulic, gentisic and p-coumaric acid), which have been previously evidented as relatively potent antioxidants, contribute to the antioxidant properties of the investigated extract. Antioxidative nature of ethanolic extract from sugar beet pulp, that is sugar beet pulp itself, indicate that it could be used as antioxidative component for decreasing oxidative changes in foods
Izvod U radu je razvijen i validiran postupak tečno-hromatografskog određivanja jonofornog kokcidiostatika salinomicina uz UV spektrofotometrijsku detekciju i post-kolonsku derivatizaciju dimetilaminobenzaldehidom (DMAB). Metoda je zasnovana na ekstrakciji salinomicina iz uzoraka hrane za životinje smešom acetonitril-voda (80:20, V/V) i prečišćavanju dobijenih ekstrakata na filteru 0.2µm Acrodisc® PSF. Relativna standardna devijacija za preciznost i obnovljivost je bila u opsegu od 2.4 do 8.8%, odnosno od 2.6 do 8.8%, a obnovljivost je bila u opsegu od 89 do 98%. Donja granica detekcije je iznosila 47 µg/kg, a donja granica kvantifikacije 71 µg/kg. Na osnovu dobijenih rezultata, zaključuje se da je opisana metoda tačna, precizna, selektivna i reproduktivna i da se može primeniti za određivanje salinomicina u premiksima i hrani za živinu.
After technological processing of rapeseed significant amounts of useful and useless waste products stand out. The aim of the present study was to investigate the chemical composition, content of glucosinolates, microbiological safety, and presence of mycotoxins and heavy element contents of useful rapeseed by-products which are intended for animal nutrition. Feed components as well as complete mixtures for animal feed must be safe and in accordance with the requirements of the current national regulation. The investigated useful by-products contained significant amounts of proteins (21.80%) and fat (33.78%). As a part of the research, extrusion of the mixture containing rapeseed by-products and maize in the ratio of 50:50% was performed. The process of extrusion was carried out at 130 °C. Based on the obtained results it was noticed that the investigated extruded mixture is a convenient protein-energy supplement suitable for animal feed production with significantly reduced glucosinolate content (from 10.30 to 7.82 μmol/g). It was also observed that the extrusion of feed mixture led to the reduction of the number of microorganisms which ensures safe feedstuff for animal feed production.
The feathers gained by slaughtering fattening chickens can be processed into protein meal for feeding certain animals, as indicated by its chemical characteristics. However, raw feather proteins (keratin) are faintly digestible (cca. 19%), even inert in digestive tract. Digestion of feather proteins could be improved by hydrolysis (alkaline, enzymatic, microbiological or hydrothermal). Practically, hydrothermal processing of raw feathers is mostly applied. The influence of hydrothermal processing under the pressures of 3.0, 3.5 or 4.0 bar on the nutritive value of the resulting meal is presented in this paper. For the hydrolysis of raw feathers, semi continuous procedure was applied. Semi continuous procedure of feathers processing comprise hydrolysis of raw wet feathers followed by partial drying of hydrolyzed mass that has to be done in a hydrolyser with indirect heating. Continuous tubular dryer with recycled air was used during the final process of drying. Protein nitrogen decreased by 3.46% and 4.80% in comparison with total protein nitrogen content in raw feathers under the pressure of 3.0 and 3.5 bar, respectively. The highest applied hydrolysis pressure caused the greatest loss of protein nitrogen up to 9.52%. Hydrothermal hydrolysis under pressure has increased in vitro protein digestibility significantly. Under pressure of 3.0, 3.5 and 4.0 bar digestibility of proteins increasing from 19.01 to 76.39, 81.71 and 87.03%, respectively. Under pressure of 3.0, 3.5 and 4.0 bar cysteine content decreased from 6.44 to 4.17% (loss 35.25%), 3.94 (loss 38.825%) and to 3.75% (loss 41.77%), respectively. These decreases are statistically significant. It can be concluded that the hydrolysis carried out under the pressure of 3.5 bar, during the period of 25 minutes, and with the content of water in raw feathers of cca. 61% is the optimal technological process for converting raw feathers into diets for certain animal diets
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