A rapid and sensitive reverse phase high performance liquid chromatography (RP-HPLC) was developed and validated for the analysis of valacyclovir hydrochloride in bulk and pharmaceutical dosage form. A phenomenex C18(250mm×4.6mm, 5µm) provided chromatographic separation using methanol:water (60:40), pH(3.5) adjusted with glacial acetic acid at the flow rate 0.8ml/min with UV detection at 251nm.valacyclovir hydrochloride was eluted at 2.24mins.The method was validated for linearity, precision, accuracy, robustness and recovery. The method was linear in the concentration range of 25-150µg/ml with correlation coefficient 0.9998. limit of detection and limit of quantification were found to be 0.000124µg/ml and 0.0003759µg/ml respectively.