No abstract

Tumor-infiltrating lymphocytes (TILs) in breast cancer are a key representative of the tumor immune microenvironment and have been shown to provide prognostic and predictive biomarkers. The extent of lymphocytic infiltration in tumor tissues can be assessed by evaluating hematoxylin and eosin (H&E)-stained tumor sections. We investigated tissue microarrays of 31 invasive breast cancer patients, looking at quantity and topological distribution of CD3+, CD8+, CD20+, Ki67+, FoxP3+ TILs and CD3+/FoxP3+, CD8+/FoxP3+ cell ratios. We separately evaluated TILs at the invasive edge and at the center of the tumor, to find any clinical implications of tumor heterogeneity. No statistically significant difference was found in quantity and distribution of both TIL subsets and TIL ratios, by comparing patients who suffered from a local or distant recurrence of the tumor (relapse group: 13 patients) with patients not showing cancer relapse (non-relapse group: 18 patients). In the whole sample, we observed three main statistically significant positive correlations: (1) between CD3+ and CD8+ T-cells; (2) between FoxP3+ and Ki67+ lymphocyte infiltration; (3) between CD3+/FoxP3+ cell ratio (C3FR) and CD8+/FoxP3+ cell ratio (C8FR). Tumor heterogeneity and stronger positive TIL associations were found in the non-relapse group, where both CD3–CD8 and FoxP3-Ki67 inter-correlations were found to be significant at the center of the tumor, while the correlation between C3FR and C8FR was significant at the invasive edge. No correlations between TIL subsets were detected in the relapse group. Our findings suggest the existence of stronger inter-subtype lymphocytic networks in invasive breast cancer not showing recurrence. Further evaluations of clinical and topological correlations between and within TIL subsets are needed, in addition to the assessment of TIL quantification and distribution, in order to follow up on whether morphological evaluation of TILs might reveal the underlying lymphocytic functional connectivity and help relapse prediction.

M. Wiedermann und O. Charamza 7 Abbildungen os de,-Radioisolopenobledung (Voistand: ¡)r. ,m'd. M. Tliedermonn) des f/n i,'e,sitälskrankenhw, ses Oloinouc/OSSU iiiul dem Zeniralinslitul fur Kernforschung, flosseiidorf (I)irektor: Piof. Dr.-Ing. If. Faulstich) Heruntergeladen von: NYU. Urheberrechtlich geschützt.

No abstract

Assuming that colloidal particles of ls*Au or goy are adsorbed by the synovial surface so that they form a very thin plane source of activity, the dependence of the absorbed dose on the distance from the source is calculated according to Berger's method and the methods of Loevinger et al. and Cross. Considering an infinitely thin plane source, a model often used in the literature, excellent agreement among the three methods is obtained at distances above 0.05 mm, A plane source having a thickness of 20-30 pm appears to be a more reasonable model, approximating more closely to the actual distribution of colloidal particles in the synovial membrane. These two approaches are compared and shown t o give the same dose at distances greater than 0.1 mm. IntroductionRadioactive colloids, mainly 198Au and 9OY, are often used for the treatment of some joint conditions (