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e24813th International Congress on Infectious Diseases Abstracts, Poster Presentations ination and acid-fast staining, they had been cultured in Lewenstein-Jensen medium. After isolation of mycobacteria by differential tests such as niacin, nitrate reduction, arylsulfatase, catalase, urease, pyrazinamidase, susceptibility to TCH, tween hydrolysis, tellurite reduction, tollerance to 5% NaCl, rate of growth and pigmentation (dark-light), their antibiotic resistance and susceptibility were studied. Other bacteria were identified by staining and culturing in different media as standard methods.Results: Among 88 samples, 3 mycobacteria isolated (3.4%); out of these 3 proved agents cases. 1 M. chelonae (Rapidly growing) 2 cases M. scrofulaceum Antimycobacteria susceptibility test to INH, SM, RMP EMB, KM, THA were done. E. coli (41% 0 and S. Coagulase negative (38%) were isolated.Conclusion: Since atypical mycobacteria exist in soil, and some of cases from these bacteria have been isolated in Iran, therefore, isolation of them from person especially children after a disaster such as earthquake is important.