Anaerobic digestion is a biological process in which the organic material is converted by microorganisms to methane and carbon dioxide (biogas) in the absence of oxygen. This process is interesting but the control on industrial scale spontaneous biological reactions requires good knowledge of the phenomena involved. The search for appropriate models to be use in control theory is now a high priority to optimize fermentation processes and solve important problems, such as the development of renewable energy from biodegradable organic waste. The aim of this study is modeling of biokinetics of anaerobic digestion on several aspects such as microbial activity, substrate degradation and methane production. For this, we developed a mathematical model based on mass balances on biomass, the organic substrate and biogas. This model is then simulate on Matlab using the experimental data from the literature and comparison between other models and our experimental results. The sensitivity of the model to the process parameters was study by varying the initial concentration of the biomass and the dose of the organic substrate.

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Economically and technically interesting, the bioreactor phenomena are studied on both micro (pore and particle size) and macro (bioreactor) levels, to describe synergetic between bio-dynamics and physicochemical dynamics. Still, the three-phase bioreactors modelling remains complex. It requires taking into account numerous factors: the pollutant biodegradation rate in the biofilm, the reactant interfacial gas-liquid and liquid-solid mass transfer, the biofilm composition and growth, the granular structure and all the transfer phenomena occurring between phases in presence. The aim of this paper is to present a model based on biofilm composition and growth, the fluidized bed structure and its hydrodynamics. The simulation results are compared with our experimental data obtained for a biological treatment, carried out in three-phase fluidized bed reactor for hydrocarbons removal from refinery wastewaters. The equipment used is laboratory scale and the obtained results show that we could degrade hydrocarbons efficiently.

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