Highly pathogenic avian influenza (HPAI) A(H5N1) viruses have been circulating since 2003 in Indonesia, with major impacts on poultry health, severe economic losses, and 168 fatal laboratory-confirmed human cases. We performed phylogenetic analysis on 39 full-genome H5N1 virus samples collected during outbreaks among poultry in 2015–2016 in West Java and compared them with recently published sequences from Indonesia. Phylogenetic analysis revealed that the hemagglutinin gene of all samples belonged to 2 genetic groups in clade 2.3.2.1c. We also observed these groups for the neuraminidase, nucleoprotein, polymerase, and polymerase basic 1 genes. Matrix, nonstructural protein, and polymerase basic 2 genes of some HPAI were most closely related to clade 2.1.3 instead of clade 2.3.2.1c, and a polymerase basic 2 gene was most closely related to Eurasian low pathogenicity avian influenza. Our results detected a total of 13 reassortment types among HPAI in Indonesia, mostly in backyard chickens in Indramayu.

Indonesia has a potency to produce its own propolis, however the propolis market in Indonesia is dominated by imported product, such as from Brazil. Currently, still there is no reasearch which evaluate bioactive compound and nutrient content of Indonesian Propolis (IP) compare with Brazilian Propolis (BP). The objectives of this study were to analyze bioactive compounds and nutrient contents of IP compared to BP. Bioactive compounds and nutrients content were analyzed by gas chromatography–mass spectrophotometry. The results<br />showed both IP and BP contain fenol, α-amyrin, cylolanost, and pyrimidines. Bioactive compounds which specifically found in IP were eudesmane compound, ethyl acridine, lupeol, friedooleanan; while β amyrin and cinnamic acid compound only found in BP. The nutrient contents of IP were higher than BP except for vitamin A. In conclusion, IP might have potential health benefit, similar to BP.<br /><br />

Curcuma aeruginosa is the common name of temu hitam in Indonesia, and the rhizome parts of the plant have several pharmacological activities. Generally, pharmacological activities are associated with bioactive content in the extract of medicinal plants. Several factors can in luence the bioactive extraction from medicinal plants such as solvent types, extraction time, extraction technique, and liquid-to-solid ratio. In this research, the extraction factors for extraction yield and cytotoxic activity of C. aeruginosa rhizome were optimized using the Box-Behnken experimental design. Effect of ethanol concentration, the ratio of liquid to solid, and extraction time for the maceration process was studied. The cytotoxic activity was determined by the brine shrimp lethality test. The optimum value that maximizes the extraction yield was 70% ethanol, 300:15 ml/g liquid to solid ratio, and 1-day extraction time. The optimum value that maximizes the cytotoxic activity was 70% ethanol, 150:15 ml/g liquid to solid ratio, and 2-day extraction time. The predicted extraction yield and cytotoxic activity at these projected values are 14.78% and 78.26 mg/l, respectively. In this model, Adeq Precision (10.35 and 4.16), R-Squared (0.86 and 0.79), and F-value (7.92 and 2.04) is rational to it the model for extraction yield and cytotoxic activity from C. aeruginosa rhizome.