A urea-functionalized chemoreceptor 1,5-bis(2,4dinitrophenyl)carbonohydrazide (BDC) with versatile applications has been reported in this work. BDC displayed ditopic sensitivity toward toxic industrial pollutants Cu 2+ and CN − from a purely aqueous medium. BDC has been structurally authenticated by ESI-MS, 1 H-NMR, FT-IR, and SCXRD. It can undergo promising "naked eye" detection in the existence of the targeted analytes (pale yellow to dark purple for Cu 2+ and pale yellow to dark brown for CN − ) in the sub-nanomolar detection threshold (Cu 2+ : 46 × 10 −8 M and CN − : 92 × 10 −8 M). The LMCT-ICT and intermolecular H-bonding pathways rationalize the underlying sensing mechanism. A good substantiation of solution-state experimental outcome and theoretical (DFT) evidence further authenticated the recognition pathway. BDC displays reversibility with alternate CN − and Cd 2+ addition up to several cycles that serves to be a reliable system toward mimicking molecular logic gate functions. The cytotoxicity assay of BDC on Bacillus thuringiensis (Bt) exhibit its biocompatibility. Furthermore, BDC can efficiently undergo rapid on-site Cu 2+ and CN − detection in varying real water sources, and interestingly, BDC can recognize the presence of Cu 2+ from biofluids like fetal bovine serum (LOD 13 μM) with a distinct colorimetric response. Moreover, the unprecedented novelty of biocompatible BDC lies in its ability to detect Cu 2+ from a human urine specimen as low as 1.5 μM by concentration-dependent discriminative chromogenic responses. This makes BDC an inimitable exploratory symptomatic tool for Wilson's disease, which to the best of our knowledge to date has been rarely explored in the supramolecular realm. One step ahead, solid-state on-spot chromogenic detection of aqueous Cu 2+ and CN − by the "dip stick" method escalates the practical application of this work.

A ratiometric chemosensor (DNMH) is unveiled herein, demonstrating selective chromogenic response towards CN−, with a LOD of 278 nM. Consequently, molecular logic circuitry and a smartphone-based colorimetric sensory prototype has been explored.

In recent times, trimethylamine N-oxide (TMAO) a gut metabolite generated by constitutive oxidation and reduction by gut microbial and host enzymes is gaining increased attention of scientists as it has been linked to the development of atherosclerosis and other ailments such as chronic kidney disease, type 2 diabetes mellitus, etc. TMAO which acts as a biomarker for health risk is generated by the oxidation of trimethylamine (TMA), produced by human gut microflora from proteinaceous food material. Microbial degradation of TMA can be a predicted approach towards the reduction of the effect of TMAO on human health. The isolated Paracoccus sp. strain PS1 could rapidly grow in mineral salt medium supplemented with TMA as the sole source of carbon and nitrogen. Its TMA degrading capacity was further confirmed through spectrophotometric, Electrospray Ionization Time-of-Flight Mass Spectrometry (ESI TOF-MS) and High performance liquid chromatography (HPLC) analysis. In silico analysis of the TMA/TMAO degrading enzymes were performed using bioinformatics tools.