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The dietary requirement of juvenile Asian sea bass Lates calcarifer Bloch for total sulphur amino acids was studied. Fish (average initial weight of 2.59 ± 0.08 g) were reared in twelve 500 L fibreglass tanks provided with flow‐through seawater at 26°C and salinity of 31 p.p.t. for 12 weeks. They were fed semi‐purified test diets containing 6.2, 7.2, 8.1, 9.0, 10.8, or 12.6 g methionine kg−1 dry diet and a basal level of 3.1 g cystine kg−1 dry diet. The mean crude protein of the diets (containing defatted Peruvian fishmeal, squid meal, soybean meal, and free amino acid mixture to simulate the pattern of hydrolysed sea bass protein) was 46.02%. The crude fat content of the diets was 10.51% from a 1 : 1 mixture of cod liver oil and soybean oil. Survival was 100% in all treatments. On the basis of the growth response, the total sulphur amino acid requirement of juvenile Asian sea bass was estimated to be 13.4 g kg−1 dry diet (2.9% of protein). Fish fed low levels of l‐methionine had significantly lower weight gains and feed efficiency ratios as well as slightly higher hepatosomatic indices. No nutritional deficiency signs were observed other than growth depression in fish fed on diets that were low in methionine. This information is valuable in further refinement of formulations of practical diets for the Asian sea bass.

The approximate levels of dietary protein and energy that would sustain good growth and survival of the mangrove red snapper Lutjanus argentimaculatus (Forsskal) were determined in two feeding experiments. In the preliminary experiment, six fish meal‐based diets were formulated to contain three protein levels (35%, 42.5% and 50%) and two lipid levels (6% and 12%) for each protein, with dietary energy ranging from 14.6 MJ kg−1 to 20.5 MJ kg−1. The protein to energy (P/E) ratios of diets ranged from 20.6 mg protein kJ−1 to 27.5 mg protein kJ−1. Diets were fed for 100 days to triplicate groups of snappers with an average initial weight of 24.8 ± 0.4 g. No significant interaction between different levels of protein and lipid was observed. Survival rates (93.8% to 100%), feed conversion ratios (FCR) (2.61–3.06) and condition factors (K) were not affected by different dietary treatments. Regardless of lipid level, fish fed 50% protein diets had a significantly higher specific growth rate (SGR) than fish fed the 35% protein diets, but not compared with the 42.5% diets (P < 0.05). Increasing lipid to 12% in all protein levels resulted in no improvement in growth over the 6% level. Fish body moisture did not vary while lipid levels based on dry matter were high (27.9% to 33.7%). Snapper appear to require more than 40% dietary protein and a high dietary energy level for good growth. In the second experiment, fish (21.1 ± 0.1 g) in four replicate groups were fed for 94 days with three diets (39%, 44% and 49% protein with P/E ratios of 21.1, 23.3 and 25.5 mg protein kJ−1 respectively) containing similar dietary energy levels of about 19 MJ kg−1. Average final weight, SGR and FCR were significantly higher in diets containing 44% and 49% protein diets (P > 0.05). There were no differences in survival rates, protein efficiency ratio (PER) and nutrient composition of snapper flesh. All fish had fatty livers. Results indicated that the diet containing 44% protein with a P/E ratio of 23.3 mg protein kJ−1 was optimum for snapper growth under the experimental conditions used in the study.

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