Introduction: Gnetum gnemon L. (Melinjo) is popular in Indonesia as a source of nutrition. Its seeds contain trans-resveratrol, which has been shown to have multiple pharmacological activities. The application of ionic liquid [Bmim]Br as a selected solvent in the microwave assisted extraction (MAE) method was developed for extraction of trans-resveratrol from Melinjo seeds. MAE method with ionic liquid has also been applied to extract trans-resveratrol from the different plant with a high extraction yield value. Methods: The Box-Behnken design on four factors with response surface methodology were used to optimize extraction conditions for transresveratrol content from Melinjo seeds. For further, the various salt was applied to separate of the trans-resveratrol form ionic liquid and facilitate for extraction back using an organic solvent. Results: The optimum process for the ionic liquid-based microwave assisted extraction was below: [Bmim]Br concentration 2.5 mol/L; liquid-melinjo seeds powder ratio 15 mL/g; microwave power 10%; and extraction time 10 min. Under optimized conditions, the trans-resveratrol value was 1.34 mg/g. Moreover, the salt Na 2 CO 3 0. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.