The present work was directed to study the effect of bio and inorganic fertilizer treatments on the ultra structure antennae of female and male silkworm (Bombyx mori L.), as a trail to understand the effect of the type of fertilization on mulberry trees and silkworm antennal sensilla. The sensory organs on the antennae were visualized by scanning electron microscopy. The results showed that the surface of the antenna is covered with scales overlapped together and is provided with sense sensilla that found on the ventral and lateral surface of the antenna. On the silkmoth antennae, six different types of sensilla have been identified: Olfactory sensilla trichodea, s. basiconica, s. coeloconica, putative gustatory s. chaetica that are multimodal receptors, s. styloconica, that are thought to be thermo-and hygroreceptors and the mechano-sensory sensilla (squamiformia). Silkworm (B. mori) is a monophagous insect that drives almost all required nutrients for its growth and development from mulberry leaf. Mulberry trees were treated with bio fertilizes, chemical fertilizers and the mixture of them through the addition in soil. The results of chemical fertilizer, bio-fertilizer and its mixture increased the growth, development, health, feed consumption and larval weight, total larval duration and this reflected on the number and length of sensilla. Chemical fertilizer, biofertilizer and its mixture increased the size of sensilla than control. Moreover, application by bio-fertilizer resulted in better values larval weight, total larval duration and the size of sensilla. Both chemical and bio fertilizes exhibited better values for pupal weight which reflect on the size of sensilla.
Pollen grains are virtually the main source of protein, fats, vitamins and minerals in the diet of the honeybee, necessary for normal growth and development. The present study is concerned with the effect of pollen contaminated with the heavy metals cadmium, chromium and the acaricide Amitraz on the hypopharyngeal glands development, growth (changes in the dry weight of thorax) and worker longevity. The obtained results showed that feeding the newly emerged workers on the control candy patty (candy + 10 % pollen) for 10 days promoted the hypopharyngeal glands development and the mean dry weight of thorax to the highest level compared with those bees fed on candy patty contaminated with various concentrations of both cadmium and chromium (0.5, 1, 3, 5, and 10 ppm). Statistical analysis showed that, no significant differences were found between the experimental diets, except chromium at the 10 ppm concentration which significantly decreased the hypopharyngeal glands development and the mean dry weight of thorax .Concerning the longevity of workers fed on the experimental diets contaminated with chromium and cadmium, the statistical analysis showed no significant differences between all the tested diets. With regard to the effect of the acaricide (Amitraz) on bees fed on diets contaminated with any concentration of (0.05, 0.1, .05, 1.5 and 3 ppm.). all of these concentrations failed in promoting the development of the hypopharyngeal glands. For, the mean dry weight of thorax and the longevity of workers fed on Amitraz, the statistical analysis showed no significant differences were found between the experimental dites.
The present investigation reports the effect of folic acid as supplemented feed on haemogram and some biological parameters of Bombyx mori L. All the tested concentrations of folic acid especially 10mg/100ml significantly increased the weight of larvae ,pupae, fresh cocoon and cocoon shells. Also, treatment of folic acid increased total haemolymph protein, total haemocyte count, differential haemocyte count and corpora allata activity.
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