Objective The aim of the present study is to evaluate the urinary KIM-1 level in the patients after 24 h angiography as a predictive biomarker of contrast-induced acute kidney injury. Methods This study included 80 selective patients attending in the cardiology unit (48 males, 32 females). The study was conducted in the cardiac catheterization unit at Al-Hussein Medical City/ Kerbala. Clinical examination and laboratory investigations were made before and 24 h after angiography, these investigations include: serum creatinine, blood urea and estimated GFR. Urinary KIM-1 was measured before and after 24 h angiography. Results There was no significant difference in urinary KIM-1 when compared between CIN and non CIN group P > 0.05. The level of urinary KIM-1 increased in the patients after 24 h of angiography when compared with baseline level of P < 0.001. Conclusion Urinary KIM-1 was not useful for predicting or detecting CIN. But urinary KIM-1 level may be useful as a biomarker for tubular damage following intravascular administration of contrast media, 24 h.