ontamination of aquatic ecosystems with heavy metals has increased worldwide. Heavy metals are interesting and noteworthy due to their strong impact on aquatic ecosystems and their bioaccumulation in hydrobionts. Cadmium and Copper were tested in genotoxicity assays with some contradictory results (Ayllon and Garcia, 2000; Conners and Black, 2004). Metal accumulation causes an increase in highly reactive oxygen species (ROS) such as hydrogen peroxide, superoxide radical, hydroxyl radical which leading to oxidative stress in fish (Dautremepuits et al., 2002). Many factors including heavy metals in soil and waste water (Yu, 2000) can affect the DNA genetic material of organisms directly or indirectly and not only to damage the integrity of the DNA structure but also influence its expression and eventually cause genotoxicity to organisms. Copper sulfate is one of the pesticides that can be troublesome. The most copper sources are the agricultural fertilizers and pesticides and the frequent cause of poisoning in aquatic ecosystems. Copper compounds are also found in preservatives, additives and coloring