BACKGROUND Quality of Life is a personal interpretation about the life status in the context of culture and value system. The quality of life of midwives working in the health care system requires special attention. The present study was conducted with an aim to investigate the quality of life of midwives working in clinics and maternity hospitals in northwest of Iran. MATERIALS AND METHODS This cross-sectional study was conducted with participation of 70 midwives working in health centers and maternity hospitals in Maragheh. The data collection tool in the present study was Quality of Life Quality Questionnaire of the World Health Organization (WHOQOL-BREF-26). The collected data was analysed using software SPSS version 16 and Kruskal-Wallis, Mann-Whitney and Pearson correlation descriptive tests. The significance level was considered 0.05. RESULTS The mean age of the subjects was 31.18 years with a standard deviation of 7.74. The mean and standard deviation of the overall quality of life score was 74.54±17.06 from 100. The highest and lowest scores were related to the domain of the physical health (71.34±14.14) and environmental health (61.77±14.87), respectively. A significant difference was found between the mean scores of the domains of the quality of life and employment type. Spearman correlation test showed that a statistically significant correlation was found between the physical health, mental health and total score of quality of life and age. CONCLUSION The results of the present study showed that the average quality of life score of subjects was higher than mean and was moderate. It is suggested that midwives receive training on the factors affecting the quality of life to become empowered to enhance their quality of life and the health of themselves and their clients.