The main aim of the research is survey of relationship between personality characteristics with academic staffs' job satisfaction and mental health in the University of Guilan. The statistic population was all these academic staffs in the year of 2014 (500). 196 individuals have selected by Kerjecy & Morgan (1970) and random sampling method. The research instruments were personality characteristics (NEO), General Health Questionnaire (GHQ) and self-made questionnaires. Pearson's Correlation, Multiple Regression and Multiple Variant (Manova) have used for analyzing data. Results showed that there was a relationship among neurotic and settlement's personality characteristics with job satisfaction and neurotic, openness, extroversion personality characteristics and settlement with mental health. Some of personality characteristics had been anticipators of mental health and job satisfaction and personality characteristics with mental health and job satisfaction had differenced depend on sociologic variances relationships.