Introduction: Marburg viruses are a group of negative-stranded RNA virus. It was first identified in 1967 during a small outbreak. During that outbreak, the fatality rate increased highly and so many people were died by the Marburg virus. Later seven strains of Marburg virus were identified from those infected humans. This virus causes Marburg virus disease (MVD) in human referred to as Marburg hemorrhagic fever. Marburg virus is endemic only to Africa; however, there have been outbreaks in Europe and the U.S.A. in recent time. Objective: However, the Marburg virus has a high fatality rate, so a preventive measure should be taken to prevent infection. As there is no effective therapeutic agent available against these viruses, effective vaccine design touching all strains would be a great step for human health Methods: In our recent study, we used in silico analysis for designing a novel epitope-based vaccine against all strains of Marburg virus. As it consists of several structural proteins and multiple sequence alignment (MSA) of Glycoproteins, RNA-directed RNA polymerases, Nucleoproteins, Vp24 proteins, Vp30, Vp35 and Vp40 proteins showed all strains of Marburg virus were conserved in RNA-directed RNA polymerase proteins. Using that protein’s conserved region, T-cell and B-cell epitopes were determined Results: Among the predicted epitope, only TIGNRAPYI was found to be highly immunogenic with 100% conservancy among all strain of human Marburg virus. The Analysis also showed both types I and II major histocompatibility complex molecules interact with this epitope and found to be nonallergenic too. Conclusion: In vivo study of the proposed peptide is suggested for novel universal vaccine production that might be an effective way to prevent human Marburg virus disease.

The shrimp white spot syndrome virus (WSSV) causes significant damage to aquaculture production worldwide but a vaccine, eliciting the immunogenicity of shrimps against WSSV has yet to be developed. Thus, a programmed immunoinformatics study was conducted to find out the potential immunogens based on genome-wide screening of WSSV envelope proteins. The measurements of the phylogenetic and evolutionary distances found the common geographical routes of three countries, where the proteins from other six countries were clustered together. Among all the four major envelope proteins i.e., VP19, VP24, VP26, and VP28; AAO69663.1 from VP26 showed the highest antigenicity and thus selected for further studies. The properties of the secondary and tertiary structure including the modelled 3D protein revealed that the protein had all the properties required for a protective immunogen. The peptide regions ranging from 99 to 115 and 98 to 106, representing the sequences "VTAPRTDPAGTGAENSN" and "TVTAPRTDP" were found to be most effective regions for B-cell linear and cytotoxic T lymphocyte (CTL), respectively. The CTL epitope also showed a strong and stable interaction with the MHC class I and class II molecules, reported to be found in fish. Therefore, the present epitope could be used as a potential vaccine candidate against WSSV.

In this study, methanolic crude extracts of Stephania japonica (Thunb.) Miers. (MESJ) whole plants were examined for possible antidepressant and sedative-hypnotic activities. Herein, the forced swimming test and tail suspension test were conducted to explore the antidepressant activity. In addition, the open field test and hole-board test were performed to evaluate the sedative-hypnotic activities. In the acute toxicity test, the MESJ ensured safety up to a dose of 2000 mg/kg, p.o. The experimental doses were 100 and 200 mg/kg p.o. In both the forced swimming test and tail suspension test, the extract significantly (p<0.01 and p<0.05) inhibited immobility time in a dose dependent manner compared to the control. These results (13.56-26.46% inhibition) indicate the mild antidepressant activity of MESJ compared to nortriptyline (60.4-64.6% inhibition). The open field test and hole-board test demonstrated the dose dependent significant (p<0.001, p<0.01 and p<0.05) and moderate sedative-hypnotic activities of the extract compared to diazepam. However, these activities were found to gradually decrease after 60 min in the open field test and must be considered as short-term activities, compared to diazepam. It can be claimed that the methanolic crude extract of Stephania japonica possesses mild antidepressant and moderate but short-term sedative-hypnotic activities.

: Medicinal plants are traditionally familiar to treat various physical abnormalities, diseases and illness throughout the world. A very large number of plant wealth has been offered by the nature for all living organisms, which preserves medicinal excellence. Traditionally in the rural areas folk medicinal practitioners perform a more ordinary manner of medicine, where medicinal plants constitute the foremost and most often only components of formulations. Geographical and cultural factors of Bangladesh create it an abundant source for herbal remedies. Nowadays, several medicinal herbs having their hypnotic and sedative effects are thoroughly used in the treatment of various psychiatric related disorders that include anxiety and insomnia. Sedatives are that types of drugs which diminish the action, inducing a calming and relaxing outcome. Sedatives, in general, produce sleep at higher doses. From recent years the prevalence of psychiatric disorders which include anxiety and insomnia is rising and therefore different researches are going on to reveal better medicine to treat these disorders. In this present review, we have performed a comprehensive literature search to find out the five most frequently used medicinal plants with sedative effects for the treatment various disorders like anxiety and insomnia and their pharmacological activities in scientific researches. The featured plants of this review articles are, Kaempferia galanga, Cleome Rutidosperma, Kalanchoe pinnata, Calotropis gigantea, Scoparia dulcis L. In herbal and traditional medicines, numerous plants are used in the absence of their scientific validation and we intend to carry out a literature review in order to find out the effective scientific value of the featured plants. This study will help to affirm the uses of these plants as traditional medicine and for researchers to detect the efficient therapeutic drugs according to their pharmacological studies.