The study included the genotoxic effects of lead and cadmium on DNA damage of 60 workers of some fuel stations in Hilla city. The results revealed a significant differences (P<0.05) of lead and cadmium, the concentrations of Pb+2 in blood of control (mean ±SD) was 6.3±0.41 μg/dl while in blood of workers who exposed to fuel for one year, three years and five years were significantly increased and reached (15.5±2.46, 27.4±11.6 and 44.5±17.8) μg/dl respectively. Cadmium concentrations in blood of control was nil while in workers were significantly increased (5±0.72, 12.2±2.6 and 15.3±4.21) μg/dl respectively. Damage criteria was increased significantly (P≤ 0.05) with time of exposure to fuel in comparison with control.