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Bloodstream infections (BSIs) are the leading cause of morbidity and mortality in people of all ages. On time diagnosis and appropriate antimicrobial treatment is of utmost important to save the lives of affected ones. The present study is aimed to determine the bacterial profile and antimicrobial susceptibility patterns of blood culture isolates among BSI suspected patients attending in Uttara Adhunik Medical College and Hospital from 1st July to 31st December, 2020. This cross sectional study was conducted among 1,675 BSI suspected patients. About 10 ml of venous blood for adults and 2-3 ml for children was collected aseptically and transferred into an automated blood culture bottle. The BD BACTEC FX40 automated blood culture method was used to isolate bacterial pathogens and antimicrobial susceptibility test was performed by Kirby-Bauer disc diffusion method following CLSI guidelines. The rate of bacteriologically confirmed cases was 222/1675 (13%). Majority of BSI were caused by gram negative bacteria predominantly Salmonella Typhi (51%) followed by Salmonella paratyphi (15%), Escherichia coli (15%), Klebsiella spp. (7%), Staphylococcus aureus (6%), Pseudomonas aeruginosa (4%). Salmonella Typhi and Salmonella Paratyphi isolates showed 100% susceptibility to meropenem and ceftriaxone. A higher percentage of strains of Salmonella Typhi (80%,84%,85%) and Salmonella Paratyphi (93%,95%,95%) were found sensitive to amoxiclav, chloramphenicol and cotrimoxazole whereas only 4% isolates were sensitive to ciprofloxacin which is an alarming situation. None of the antibiotic was 100% effective against Escherichia coli, Klebsiella spp. and Pseudomonas aeruginosa. Amikacin and meropenem were found more effective against gram negative bacteria. In this study, none of the S. aureus strains showed resistance to cloxacillin, amoxiclav, gentamicin and doxycycline and sensitivity to azithromycin and clindamycin were found 40% and 50% respectively. Multidrug resistance among blood stream isolates are increasing in a threatening way which need to be addressed through effective surveillance and infection control strategies. Bangladesh J Med Microbiol 2021; 15 (2): 5-11

Background and objectives: Wound infection is one of the most important causes of morbidity and mortality worldwide and antibiotic resistant bacteria are a great part of complications in treatment of the infection. The present study was conducted to isolate and identify the etiological agents of wound infection and to assess the antimicrobial susceptibility pattern of the isolates. Methodology: The retrospective study was carried out on wound infection suspected patients for six months duration. The collected pus specimens were first observed macroscopically then streaked on MacConkey agar, blood agar and incubated at 35°C for 24 to 48 hours. The isolated bacteria were identified by macroscopic and microscopic observations and biochemical reactions. Antibiotic susceptibility pattern of the isolates were assessed by Modified Kirby Bauer disc diffusion technique. Results: A total of 146 pus samples were collected; of which 84 (57.5%) showed bacterial growth. Out of a total 84 bacterial isolates; 47 (55.95%) were gram negative and 37 (44.05%) were gram positive bacteria. Staphylococcus aureus (42.86%) was the most common bacteria followed by Pseudomonas spp. (25%), Escherichia coli (15.48%), Klebsiella pneumoniae (5.95%), Proteus spp (4.76%), Enterobacter spp (3.57%) and Streptococcus spp. (1.19%). S.aureus isolates were sensitive to Linezolid (100%), Rifampicin (100%), Doxycycline (86.11%), Clindamycin (83.33%) and Cloxacillin (75%). Low sensitivity of S.aureus to Ciprofloxacin (33.33%) and Azithromycin (33.33%). Among gram negative isolates Pseudomonas aeruginosa were found highly sensitive to Imipenem (93.54%), Piperacillin-Tazobactum (87.09%), Cefepime (80.64%) and Amikacin (77.42%). Low sensitivity of Pseudomonas aeruginosa to Aztreonam (35.48%) and Ceftazidime (51.61%). Isolated Escherichia coli were highly sensitive (98%) to Imipenem, Amikacin and showed lowest sensitivity to almost all of the other drugs. Conclusion: By this study it is recommended, culture of wound swab and antibiotic susceptibility testing should be done before starting antibiotics, which will guide medical practitioners for empirical treatment of wound infection, so as to reduce the spread of drug resistant bacteria. Bangladesh J Med Microbiol 2020; 14 (1): 15-19

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