Objectives: To determine the frequency of Urinary tract infection (UTI) and the antibiotic sensitivity patterns of isolated uropathogens in pregnant females among middle socioeconomic settings. Study Design: Retrospective Cross Sectional study. Setting: Jinnah Medical College Hospital Karachi. Period: From Jan 2017 to Dec 2017. Material & Methods: This retrospective study was carried out in Jinnah Medical College Hospital (JMCH) Karachi after taking ethical review board approval. All the pregnant women who were registered in antenatal clinics from January 2017 to December 2017 was included. Data was taken from medical record department and information about age, parity, gravida, miscarriages, microorganisms of the urine samples and their antibiotic susceptibility pattern was noted. Results: Five hundred and sixty-four pregnant women of age 17-44 years were registered in the hospital in antenatal clinics during the year 2017. Out of 564 females, 48 (8.50%) presented with urinary tract infection some time during their pregnancy period. Majority of patients (54.2%) were infected with Escherichia coli (E coli), followed by Klebsiella pneumonia (16.77%), Pseudomonas spp. (12.5%), Enterococcus spp. (8.3%) and Staphylococus aureus (8.3%). These etiologic agents vary in their sensitivity pattern to antibiotics. Piperacillin-tazobactam had the highest overall sensitivity of (89.6%). This was followed by Meropenem (87.5%), Nitrofurantoin (87.5%) and Fosfomycin (81.3%). Cefixime, Cefotaxime and Amoxicillin/ clavulanic acid had overall sensitivities above 50%. Conclusion: E coli was the common organisms followed by others gram negative and positive organisms. Enteroococus spp. was also a significant bacterial isolates in this settings. Meropenem, Piperacillin-tazobactam, Nitrofurantoin, Fosfomycin were the highly effective antibiotics against isolated uropathogens. Amoxicillin/clavulanic acid and Cephalosporin also had a good results.