Ovine theileriosis is a hemoprotazoan infection transmitted by tick bites which cause severe economic loss. This study aimed to survey Theileria infection of sheep in different localities of Egypt (Cairo, Giza and Al Monofia) by using microscopic and molecular detection associated with clinicopathological and pathological investigations. A total of 152 sheep blood samples were selected from farms and veterinary clinics. Microscopic examination of blood smears revealed that the incidence of Theileria infection was 21.7% while the incidence was 36.8% using PCR assay (universal Theileria spp. Primer). The use of species-specific primers showed a result of 53.6% single infection; Theileria ovis, and 46.4% mixed infection; T. ovis and Theileria lestoquardi. According to the results of PCR, animals were divided into 3 groups: Theileria negative group, T. ovis group and mixed T. ovis and T. lestoquardi group. The examination of both infected groups revealed non-significant changes between them. The hemogram revealed significant macrocytic hypochromic anemia, leukopenia, neutropenia, lymphopenia, monocytopenia, eosinopenia and thrombocytopenia in Theileria infected groups compared with Theileria negative group. Biochemical analysis revealed significant hypoproteinemia, hypoalbuminemia, total and indirect hyperbilirubinemia with elevations of AST and GGT activities with increase in concentration of BUN and creatinine while no significant changes in A:G ratio values and direct bilirubin concentration in Theileria infected groups compared with Theileria negative group. The pathological investigation revealed lymphocytic depletion and necrosis with hemorrhages in lymph node and spleen. Sequencing and phylogenetic analysis were performed by targeting the 18S rRNA gene of Theileria spp. In conclusion, according to our knowledge, this is the first report of phylogeny of T. lestoquardi infected sheep in Egypt.
Feline panleukopenia virus (FPV) causes severe disease in cats, as well as in a variety of wild carnivores including many endangered animals. Regular appearance of the virus increases the importance of evaluating the percentage of identity in relation to vaccine commercially used, detecting sequence changes related to virus virulence or tropism. In this study Fifty-seven fecal samples were collected from suspected cases of cats. 19 fecal samples were positive using Rapid FLV Ag test kits kit (VDRG FLV/FIV Ab rapid kit). Fifty-seven blood samples of the same animals were subjected for hematological examination to investigate the severity of leukopenia. Thirteen out of nineteen samples were positive with a polymerase chain reaction assay (PCR) targeting VP2 gene. Three positive fecal samples in animal showing sever and moderate leukopenia were successfully sequenced Partially. Sequence analysis results showed no variation in their sequence across all isolates when compared to the published FPV genome, which could imply that FPV appears to be in genomic stasis as compared to other Parvoviruses. Genomic stasis appears among the three Egyptian samples in this study under the GenBank accession number OP594200, OP574201, and OP574202, in addition to the high similarity of the isolates the vaccine strains of EU498680 and EU498681.No amino acid substitution was detected.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.