Ear infections (particularly, otitis media) is a prevailing and common infection in developing countries causing local damage and threatening complications. Pseudomonas aeruginosa is the most common pathogen causing chronic suppurative otitis media (CSOM) and malignant otitis externa. The objective of this study is to identify incidence of Pseudomonas aeruginosa involved in ear infections and associated biochemical parameters which may be changed. External auditory canal in rabbit ears was inoculated with (10 6) colony-forming units (CFU) of Pseudomonas aeruginosa or left as sterile controls for eight weeks. There are significantly decrease results at (P<0.05) of Interleukin-2 and significant increase results of Interleukin-9 and Tumor Necrosis Factor-alpha (TNF-α) in acute or chronic infections. Moreover, histopathological changes of ear, kidney and liver tissues were also observed. Evaluation of these parameters were needed to use of specific blocking agents to inflammatory mediators with the aim of discovering new treatment options for chronic otitis media, and to help the physicians in diagnosis these diseases in elderly stages and try to reduce the pain and suffering associated with otitis media.