Proteolytic activity of two isolates of actinomycetes, Streptomyces albolongus and Streptomyces aburaviences was investigated on the basis of their ability to hydrolyze skimmed milk casein, egg albumin and gelatin. Both isolates were found to have potential for extracellular proteases production. Effects of culture conditions for the production of extracellular protease from S. albolongus and S. aburaviensis were determined. Highest protease yield from S. albolongus was obtained after 5 days of incubation with an initial p H 7, at static state when inoculated in medium composed of 1% glucose, 2% beef extract, 0.2% yeast extract, 0.1% KH 2 PO 4 , 0.3% K 2 HPO 4 , and trace MgSO 4 .7H 2 O. Optimum incubation conditions for S. abureviences were 4 days, with an initial medium p H 8 at shaking condition (100 rpm). S. aburaviences preferred 1.5% lactose and 1.5% tryptone as a carbon and nitrogen source. Both the isolates showed maximum protease yield at 37°C. The result of the present study might be helpful for largescale production of extracellular protease from these Streptomyces spp.