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This study aims experimentally to investigate the performance analysis of R134a and alternative R516A refrigerant in two evaporator ejector cooling system (DEES) at different air velocities of evaporator#1. Firstly, the tests were carried out with R134a refrigerant under steady-state conditions at different air velocities and then repeated with low GWP R516A refrigerant. As the tests were carried out with R134a, higher cooling capacity was achieved at different air velocity values. When the air velocity value was 1.1, 1.7, 2.2, and 2.7 ms-1, the COP value obtained from the tests with R134a was 1%, 2%, 5%, and 4% higher than R516A, respectively. Additionally, test results illustrate that the higher air velocity contributed to increasing performance parameters however higher air velocity than 2.2 ms-1 had a slight effect. Based on this study, it is concluded that R516A performance is convenient to R134a performance and can be alternatively used as a refrigerant in vapor compression refrigeration systems(BSS).