Polyvinyl acetate (PVAc) nanocomposites were successfully prepared based on silver nanoparticles (Ag-NPs). Firstly, Ag-NPs was directly prepared during the in situ emulsion polymerization of vinyl acetate monomer using AgNO 3 as a source of Ag + ions and poly(vinyl alcohol) (PVA) was used for dual functions as emulsifier for emulsion polymerization and a s stabilizer agent, tri-sodium citrate (C 6 H 5 O 7 Na 3 ) was used as reducing agent for Ag + ions during the polymerization process. The prepared PVAc/Ag nanocomposites were assessed using XRD, SEM, FT-IR, TEM and UV-spectra. The antibacterial properties of the prepared PVAc/Ag nanocomposites were investigated as antimicrobial activity against pathogenic bacteria, i.e., Staphylococcus aureus (G +ve bacteria) and Escherichia coli (G -ve bacteria). These PVAc nanocomposites could be used as a promising material for enhanced and continuous antibacterial applications as coating and packaging materials.