Persistent wounds are the most problematic for the patient as well as for the health system. Skin wounds are most exposed to bacterial attacks, which not only caused wound infections but also slow the healing process. There is a dire need to develop a better wound dressing or scaffold material that can increase the wound healing process. This study involves the development of electrospun nanofibers based on Silvadur loaded polyacrylonitrile membranes. Samples were developed by using five different concentrations (2 wt%, 4 wt%, 6 wt%, 8 wt%, and 10 wt%) of Silvadur loaded in PAN solution. The developed polymeric electrospun nanofibrous membranes possess a smooth surface with an average diameter lies in the range of 152.94nm to 185.05nm. Furthermore, these fibers show excellent antibacterial activity up to 92.25% in the case of Gram-negative (Escherichia Coli) bacteria and 98.52% in the case of gram-positive