Objective: The association between thyroid disease and breast cancer risk remains unclear. A cross-sectional study was carried out to find the association between hypothyroidism, hyperthyroidism, and breast cancer. Materials and Methods: The study included 71 diagnosed cases of breast cancer patients belonging to stage III of the disease. The mean age of controls and patients was 43-45 years. Their level of thyroid hormones was estimated. On the bases of high and low levels of thyroid hormone, women were divided into group one (hypothyroidism n= 56) and group two (hyperthyroidism n=15). Levels of neutrophils, lymphocytes, and hematocrit were estimated by the SWISS MAX analyzer. The level of thyroid hormones, TSH, 8OHG, Isoprostane, and 4-HNE was estimated by the technique of ELISA. The level of MDA was estimated spectrophotometrically. Results: It was observed that body weight was high in hypothyroid females (group 1) Levels of neutrophils, lymphocytes, and monocytes were significantly higher in group 1 of the breast cancer afflicted women, as compared to the controls and group 2. The level of thyroid hormones FT3, FT4, and TSH was also higher in group 1. The level of MDA, isoprostane, and 4-HNE was significantly high in both women with hypo and hyperthyroidism as compared to controls. Conclusions: We found an increased risk of breast cancer in women with hyporhyroidism and a slightly decreased risk in women with hyperthyroidism indicating an association between thyroid function level and breast cancer risk. Key Words: breast cancer, hypo, and hyperthyroidism, MDA, HNE, and Isoprostane

Background: Thyroid hormones are able to induce proliferation of breast cancer(BC) cells and these growth promoting effects were similar to estrogen suggesting a significant small talk between the two hormones. Aims and Objectives: A cross sectional study is designed to see the Interplay of thyroid hormones (fT3 and fT4), nitric oxide and nitric oxide synthase in potentiating BC metastasis. Material and Methods: 288 consented breast cancer patients within the age range of 30-60 years and 100 age matched healthy controls were included in the study. Estimation of thyroid hormones including fT3 and fT4, vascular endothelial growth factor (VEGF), Matrix Metalloprotienase 9 (MMP-9) and inducible nitric oxide synthase (iNOS) was carried out by ELISA. Data was analyzed by SPSS 20.0. Variables of subjects and patients was compared by student ‘t’ test. The Correlation analysis was carried out by Pearson Correlation Coefficient. . Results: Increased levels of T3, T4, VEGF,NO, NOS AND MMP 9 were observed in subjects as compared to controls. Correlation coefficient showed positive correlation between fT3,NO and NOS. On the other hand, negative correlation was observed between fT4, NO and NOS. Conclusion: This study indicates a probable relationship between hyperthyroidism, NO,NOS,VEGF in the progression and metastasis of BC. Key Words: Thyroid hormones, breast cancer, nitric oxide, nitric oxide synthase, MMP 9 and VEGF.