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CoNS are gaining importance due to increase in resistance rates to betalactam antibiotics and multi drug resistance. Although specific virulence factors are not as clearly established, it seems clear that factors such as bacterial polysaccharide components, and ability to form biofilm are involved in attachment and/or persistence of bacteria on foreign materials. Biofilms usually result in persistent infections that cannot be easily resolved with standard antibiotic treatments; therefore, the biofilm formation ability and the resistance to antimicrobial therapy can be intimately related. A prospective cross-sectional study was done on purely isolated CoNS from various clinical samples from both out patients and inpatients. All the test strains were subjected to antimicrobial susceptibility testing. The ability to produce biofilm was detected by tube adherence method. Among 193 CoNS isolates 156 were from inpatients and 37 were from out patients. Methicillin resistant was seen in 80.31%. Of the total, 40.41% showed moderate biofilm formation by tube adherence method. 23.32% of isolates did not form biofilm. All the isolates from blood samples showed moderate (20/26) and strong (6/26) biofilm formation. Among non biofilm producers 66.67% were MS CoNS isolates and 33.33% were MRCoNS. 94.59% of biofilm producers were MRCoNS and 5.41% were MSCoNS. Production of biofilm was relatively more (1.16) among CoNS isolates of IPD than OPD.  As Coagulase negative Staphylocooci are exhibiting multi drug resistance and are able to form biofilm, these organisms causing a major challenge for the physicians. Hence, such problems can be prevented by detection of biofilm producers and appropriate antibiotic doses modification. The issue of antibiotic resistance among CoNS needs to be addressed through a more rational use of existing antibiotics as well as the development of new antimicrobial agents.

Bacterial Vaginosis is one of the important cause for pre-term labour which in turn can lead to infant mortality and morbidity, an easy and reliable Bacterial Vaginosis detection procedure is needed for early diagnosis and intervention to prevent adverse effects caused by bacterial vaginosis, this study provides a view on simple diagnostic techniques for early and easy detection of Bacterial Vaginosis to combat preterm labour. METHODS AND MATERIAL: 100 women (Study group) with preterm labour were screened for the prescence of Bacterial Vaginosis by performing Whiff test, wet mount preparation, estimation of pH and Grams's staining and compared with 100 women (control group) with term pregnancy. RESULTS: the study group showed Bacterial Vaginosis in 42% whereas the control group had only 18%, Gram staining in the present study revealed normal flora 19%, intermediate flora 21%, BV 42% and scanty flora 16% in the study group. CONCLUSIONS: Preterm labour is one of the major cause for infant mortality and morbidity and Bacterial Vaginosis is one the predisposing factor for preterm labour so a simple diagnostic techniques to diagnose Bacterial Vaginosis will lead to early detection of Bacterial Vaginosis which may in turn decrease preterm labour and reduce infant mortality and morbidity.