Mouhanna, A.M., O.N. Hamoudi, M.M. Mofleh and H.S. Barhoum. 2019. Genetic variability between Fusarium spp. isolates collected from Solanacious plants in Syria with emphasis on F. oxysporum infecting tomato. Arab Journal of Plant Protection, 37(4): 293-302.This study aimed to investigate the genetic variability of Fusarium spp. isolates collected from Solanacious plants (tomato, pepper and eggplant) in Syria. 20 primers for RAPD marker were used on 59 fungal isolates. Results showed that 14 primers had the ability to amplify one or multi sites of the fungal genome. The total number of amplified fragments was 123, with a polymorphism rate of 81.3%. The phylogenetic tree showed that the isolates can be divided into two main groups (G1, G2) at a genetic similarity level of 0.58. The G1 group included F. solani isolates and the G2 group included F. oxysporum isolates. When the ISSR technique was applied on 11 F. oxysporum isolates collected from a tomato plant, 12 out of 20 primers were able to amplify 110 fragments with a polymorphism rate of 52.72%. The ISSR phylogenetic tree showed that these isolates can be divided into six groups, more or less associated with their pathogenicity (0-68%). This is the first study on genetic variability of Fusarium spp. on Solanacious plants with emphasis on F. oxysporum isolates from tomato throughout Syria.