Objectives: Golf has been associated with abnormal loading conditions to the knee joint due to the repetitiveness and biomechanical requirements of the golf swing. This study seeks to evaluate the prevalence and factors attributing to symptomatic knee osteoarthritis among professional golfers in Kenya. Materials and Methods: Participants included all professional golfers in Kenya above 18 years. Golfer’s demographics and golf-related characteristics were recorded and both knee’s function assessed using the Knee Injury and Osteoarthritis Outcome Score (KOOS). Participants with a KOOS <85% in two or more parameters underwent bilateral plain knee radiographic assessment and graded according to the Kellgren and Lawrence (K&L) classification. Results: Fifty participants were recruited. All male, only two were left handed, median age was 41 years (34–49). More than half were found to have a body mass index (BMI) of greater than 25 kg/m2. The average duration of active golf participation was 16.5 years (SD = 4.2). Eighteen out of 100 knees scored <85% in two parameters of the KOOS. According to the K&L, 72% had Grade 2 and above. Duration of active golf involvement (OR – 1.114) and BMI above 25 kg/m2 (OR – 1.107) were found to be positively associated with symptomatic knee osteoarthritis although not statistically significant (P = 0.289 and 0.3481). Conclusion: Golf presents a prevalence of symptomatic knee osteoarthritis of 18% comparable to high-impact sports. The associated factors include BMI >25 kg/m2 and longer duration of active golf participation although both not statistically significant.