Extracts in organic solvents (namely methanol, ethanol, ethyl acetate and chloroform) of two medicinal plants -Achyranthes aspera and Cassia alata were evaluated for their antibacterial activities against Escherichia coli, Bacillus subtilis, Vibrio cholerae, Salmonella typhi and Staphylococcus aureus. These were carried out by taking the organic extracts of both the leaf and stem parts of the plants at a concentration of 5 mg/ml and their activities were recorded by estimating zones of inhibition as produced by disc-diffusion method on Mueller-Hinton agar media. While neither the leaf nor stem parts of A. aspera in any organic extractions showed antibacterial activity, the methanolic extracts of both the leaf and stem parts of C. alata exhibited antibacterial activity, but only to B. subtilis and S. typhi, and the corresponding MIC values of the leaf extracts were estimated as 1.25 and 1.5 mg/ml respectively. However, the ethanolic extracts of both the stem and leaf parts were found equally effective only to S. aureus (MIC= 1.25 mg/ml). The corresponding MBC values are reported.
To study the biology of Pachliopta aristolochiae on its host plant Aristolochia indica singly laid eggs on the host plant were collected from the field and reared in the laboratory under optimum conditions of temperature (28 ± 3 °C ) and relative humidity (70 ± 5% RH). Incubation period of the egg was 5.0 ± 0.6 days, larval developmental period was 11 ± 0.3 days, pre-pupal period was 0.87 ± 0.08 day, and the pupation took 12 ± 0.63 days. The length of 1 st , 2 nd , 3 rd and 4 th instar larvae were 4.0 ± 0.63, 9 ± 0.63, 22.6 ± 5.2 and 38.2 ± 4.70 mm, respectively. The feeding potential rate of 1 st , 2 nd , 3 rd and 4 th instar larvae were 11.4 ± 5.04, 29.6 ± 5.12, 51.4 ± 6.0 and 72.8 ± 4.9%, respectively. The weight of the faeces of 1 st , 2 nd , 3 rd and 4 th instar larvae were 0.012 ± 0.004, 0.047 ± 0.018, 0.0114 ± 0.023 and 0.274 ± 0.045 gm, respectively.
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